Generation and Characterization of Novel Human IRAS Monoclonal Antibodies
Joint Authors
Liu, Ying
Yao, Zhenyu
Cong, Yuwen
Li, Jin
Liu, Xiaolan
Wang, Bo
Sun, Qihong
Shan, Yajun
Su, Ruibin
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-08-10
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Imidazoline receptors were first proposed by Bousquet et al., when they studied antihypertensive effect of clonidine.
A strong candidate for I1R, known as imidazoline receptor antisera-selected protein (IRAS), has been cloned from human hippocampus.
We reported that IRAS mediated agmatine-induced inhibition of opioid dependence in morphine-dependent cells.
To elucidate the functional and structure properties of I1R, we developed the newly monoclonal antibody against the N-terminal hIRAS region including the PX domain (10–120aa) through immunization of BALB/c mice with the NusA-IRAS fusion protein containing an IRAS N-terminal (10–120aa).
Stable hybridoma cell lines were established and monoclonal antibodies specifically recognized full-length IRAS proteins in their native state by immunoblotting and immunoprecipitation.
Monoclonal antibodies stained in a predominantly punctate cytoplasmic pattern when applied to IRAS-transfected HEK293 cells by indirect immunofluorescence assays and demonstrated excellent reactivity in flow immunocytometry.
These monoclonal antibodies will provide powerful reagents for the further investigation of hIRAS protein functions.
American Psychological Association (APA)
Wang, Bo& Liu, Ying& Shan, Yajun& Yao, Zhenyu& Liu, Xiaolan& Su, Ruibin…[et al.]. 2009. Generation and Characterization of Novel Human IRAS Monoclonal Antibodies. Journal of Biomedicine and Biotechnology،Vol. 2009, no. 2009, pp.1-8.
https://search.emarefa.net/detail/BIM-512699
Modern Language Association (MLA)
Wang, Bo…[et al.]. Generation and Characterization of Novel Human IRAS Monoclonal Antibodies. Journal of Biomedicine and Biotechnology No. 2009 (2009), pp.1-8.
https://search.emarefa.net/detail/BIM-512699
American Medical Association (AMA)
Wang, Bo& Liu, Ying& Shan, Yajun& Yao, Zhenyu& Liu, Xiaolan& Su, Ruibin…[et al.]. Generation and Characterization of Novel Human IRAS Monoclonal Antibodies. Journal of Biomedicine and Biotechnology. 2009. Vol. 2009, no. 2009, pp.1-8.
https://search.emarefa.net/detail/BIM-512699
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-512699