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Molecular Cloning and Characterization of Novel Phytocystatin Gene from Turmeric, Curcuma longa
Joint Authors
Abu Bakar, Norliza Tendot
Chan, Seow-Neng
Shaharuddin, Noor Azmi
Ho, Chai-Ling
Mahmood, Maziah
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-14
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Phytocystatin, a type of protease inhibitor (PI), plays major roles in plant defense mechanisms and has been reported to show antipathogenic properties and plant stress tolerance.
Recombinant plant PIs are gaining popularity as potential candidates in engineering of crop protection and in synthesizing medicine.
It is therefore crucial to identify PI from novel sources like Curcuma longa as it is more effective in combating against pathogens due to its novelty.
In this study, a novel cDNA fragment encoding phytocystatin was isolated using degenerate PCR primers, designed from consensus regions of phytocystatin from other plant species.
A full-length cDNA of the phytocystatin gene, designated CypCl, was acquired using 5′/3′ rapid amplification of cDNA ends method and it has been deposited in NCBI database (accession number KF545954.1).
It has a 687 bp long open reading frame (ORF) which encodes 228 amino acids.
BLAST result indicated that CypCl is similar to cystatin protease inhibitor from Cucumis sativus with 74% max identity.
Sequence analysis showed that CypCl contains most of the motifs found in a cystatin, including a G residue, LARFAV-, QxVxG sequence, PW dipeptide, and SNSL sequence at C-terminal extension.
Phylogenetic studies also showed that CypCl is related to phytocystatin from Elaeis guineensis.
American Psychological Association (APA)
Chan, Seow-Neng& Abu Bakar, Norliza Tendot& Mahmood, Maziah& Ho, Chai-Ling& Shaharuddin, Noor Azmi. 2014. Molecular Cloning and Characterization of Novel Phytocystatin Gene from Turmeric, Curcuma longa. BioMed Research International،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-512701
Modern Language Association (MLA)
Chan, Seow-Neng…[et al.]. Molecular Cloning and Characterization of Novel Phytocystatin Gene from Turmeric, Curcuma longa. BioMed Research International No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-512701
American Medical Association (AMA)
Chan, Seow-Neng& Abu Bakar, Norliza Tendot& Mahmood, Maziah& Ho, Chai-Ling& Shaharuddin, Noor Azmi. Molecular Cloning and Characterization of Novel Phytocystatin Gene from Turmeric, Curcuma longa. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-512701
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-512701