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Assessment of a Standardized ROS Production Profile in Humans by Electron Paramagnetic Resonance
Joint Authors
Mrakic-Sposta, Simona
Gussoni, Maristella
Montorsi, Michela
Vezzoli, Alessandra
Porcelli, Simone
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-07-26
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
Despite the growing interest in the role of reactive oxygen species (ROS) in health and disease, reliable quantitative noninvasive methods for the assessment of oxidative stress in humans are still lacking.
EPR technique, coupled to a specific spin probe (CMH: 1-hydroxy-3-methoxycarbonyl-2,2,5,5-tetramethylpyrrolidine) is here presented as the method of choice to gain a direct measurement of ROS in biological fluids and tissues.
The study aimed at demonstrating that, differently from currently available “a posteriori” assays of ROS-induced damage by means of biomolecules (e.g., proteins and lipids) spin-trapping EPR provides direct evidence of the “instantaneous” presence of radical species in the sample and, as signal areas are proportional to the number of excited electron spins, lead to absolute concentration levels.
Using a recently developed bench top continuous wave system (e-scan EPR scanner, Bruker) dealing with very low ROS concentration levels in small (50 μL) samples, we successfully monitored rapid ROS production changes in peripheral blood of athletes after controlled exercise and sedentary subjects after antioxidant supplementation.
The correlation between EPR results and data obtained by various enzymatic assays (e.g., protein carbonyls and thiobarbituric acid reactive substances) was determined too.
Synthetically, our method allows reliable, quick, noninvasive quantitative determination of ROS in human peripheral blood.
American Psychological Association (APA)
Mrakic-Sposta, Simona& Gussoni, Maristella& Montorsi, Michela& Porcelli, Simone& Vezzoli, Alessandra. 2012. Assessment of a Standardized ROS Production Profile in Humans by Electron Paramagnetic Resonance. Oxidative Medicine and Cellular Longevity،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-512716
Modern Language Association (MLA)
Mrakic-Sposta, Simona…[et al.]. Assessment of a Standardized ROS Production Profile in Humans by Electron Paramagnetic Resonance. Oxidative Medicine and Cellular Longevity No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-512716
American Medical Association (AMA)
Mrakic-Sposta, Simona& Gussoni, Maristella& Montorsi, Michela& Porcelli, Simone& Vezzoli, Alessandra. Assessment of a Standardized ROS Production Profile in Humans by Electron Paramagnetic Resonance. Oxidative Medicine and Cellular Longevity. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-512716
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-512716