Glycosaminoglycans Modify Elastase Action In Vitro and Enhance Elastase-Induced Cell Death in Cultured Fibroblasts
Joint Authors
de Oliveira-Santos, José
Gozzo, Andrezza Justino
Cruz-Silva, Ilana
Rydlewski, Mariana
Araújo, Mariana da Silva
Nader, Helena Bonciani
Abreu Nunes, Viviane
González, Yamile González
Praxedes-Garcia, Priscila
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-11-22
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Human neutrophil elastase (HNE) has been shown to be involved on death of different cell types, including epithelial lung cells, which is related to several pulmonary diseases.
Since HNE activity may be influenced by extracellular matrix (ECM) molecules such as glycosaminoglycans (GAGs), and fibroblasts are the most common ECM-producing cells of lung connective tissue, the aim of this work was to verify if HNE can induce fibroblast death and to study the enzyme modulation by GAGs.
HNE-like activity was mimicked by using human neutrophils conditioned medium (NCM).
Heparan sulfate and chondroitin 6-sulfate reduce the enzyme activity and modify its secondary structure.
NCM reduced cell viability, and this effect was higher in the presence of those GAGs.
NCM also increased DNA fragmentation, suggesting the occurrence of apoptosis, but without influence of GAGs.
These results can contribute to the understanding of HNE modulation in physio- and pathological processes where this enzyme is involved.
American Psychological Association (APA)
de Oliveira-Santos, José& Abreu Nunes, Viviane& Cruz-Silva, Ilana& Praxedes-Garcia, Priscila& Gozzo, Andrezza Justino& Rydlewski, Mariana…[et al.]. 2011. Glycosaminoglycans Modify Elastase Action In Vitro and Enhance Elastase-Induced Cell Death in Cultured Fibroblasts. ISRN Cell Biology،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-512721
Modern Language Association (MLA)
de Oliveira-Santos, José…[et al.]. Glycosaminoglycans Modify Elastase Action In Vitro and Enhance Elastase-Induced Cell Death in Cultured Fibroblasts. ISRN Cell Biology No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-512721
American Medical Association (AMA)
de Oliveira-Santos, José& Abreu Nunes, Viviane& Cruz-Silva, Ilana& Praxedes-Garcia, Priscila& Gozzo, Andrezza Justino& Rydlewski, Mariana…[et al.]. Glycosaminoglycans Modify Elastase Action In Vitro and Enhance Elastase-Induced Cell Death in Cultured Fibroblasts. ISRN Cell Biology. 2011. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-512721
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-512721