Evaluation of Acetylcholinesterase Biosensor Based on Carbon Nanotube Paste in the Determination of Chlorphenvinphos

Joint Authors

Oliveira, A. C.
Mascaro, Lucia H.

Source

International Journal of Analytical Chemistry

Issue

Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-6, 6 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2011-05-02

Country of Publication

Egypt

No. of Pages

6

Main Subjects

Chemistry
Science

Abstract EN

An amperometric biosensor for chlorphenvinphos (organophosphorus pesticide) based on carbon nanotube paste and acetylcholinesterase enzyme (CNTs-AChE biosensor) is described herein.

This CNTs-AChE biosensor was characterized by scanning electron microscopy (SEM) and electrochemical impedance spectroscopy (EIS).

The SEM result shows the presence of CNTs and small lumps, due to the enzyme AChE, which has a type of cauliflower formation.

From EIS analysis is possible to observe increased Rtc for CNTs-AChE biosensor when compared to the carbon nanotube paste electrode for the reaction [Fe(CN)6]4−/3−.

Using a chronoamperometric procedure, a linear analytical curve was observed in the 4.90×10-7–7.46×10-6 M range with limit of detection of 1.15×10−7 M.

The determination of chlorphenvinphos in the insecticide sample proved to be in agreement with the standard spectrophotometric method, with a 95% confidence level and with a relative error lower than 3%.

In this way, the CNTs-AChE biosensor presented easy preparation, fast response, sensitivity, durability, good repeatability, and reproducibility.

American Psychological Association (APA)

Oliveira, A. C.& Mascaro, Lucia H.. 2011. Evaluation of Acetylcholinesterase Biosensor Based on Carbon Nanotube Paste in the Determination of Chlorphenvinphos. International Journal of Analytical Chemistry،Vol. 2011, no. 2011, pp.1-6.
https://search.emarefa.net/detail/BIM-512749

Modern Language Association (MLA)

Oliveira, A. C.& Mascaro, Lucia H.. Evaluation of Acetylcholinesterase Biosensor Based on Carbon Nanotube Paste in the Determination of Chlorphenvinphos. International Journal of Analytical Chemistry No. 2011 (2011), pp.1-6.
https://search.emarefa.net/detail/BIM-512749

American Medical Association (AMA)

Oliveira, A. C.& Mascaro, Lucia H.. Evaluation of Acetylcholinesterase Biosensor Based on Carbon Nanotube Paste in the Determination of Chlorphenvinphos. International Journal of Analytical Chemistry. 2011. Vol. 2011, no. 2011, pp.1-6.
https://search.emarefa.net/detail/BIM-512749

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-512749