Evaluation of Acetylcholinesterase Biosensor Based on Carbon Nanotube Paste in the Determination of Chlorphenvinphos
Joint Authors
Oliveira, A. C.
Mascaro, Lucia H.
Source
International Journal of Analytical Chemistry
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-05-02
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
An amperometric biosensor for chlorphenvinphos (organophosphorus pesticide) based on carbon nanotube paste and acetylcholinesterase enzyme (CNTs-AChE biosensor) is described herein.
This CNTs-AChE biosensor was characterized by scanning electron microscopy (SEM) and electrochemical impedance spectroscopy (EIS).
The SEM result shows the presence of CNTs and small lumps, due to the enzyme AChE, which has a type of cauliflower formation.
From EIS analysis is possible to observe increased Rtc for CNTs-AChE biosensor when compared to the carbon nanotube paste electrode for the reaction [Fe(CN)6]4−/3−.
Using a chronoamperometric procedure, a linear analytical curve was observed in the 4.90×10-7–7.46×10-6 M range with limit of detection of 1.15×10−7 M.
The determination of chlorphenvinphos in the insecticide sample proved to be in agreement with the standard spectrophotometric method, with a 95% confidence level and with a relative error lower than 3%.
In this way, the CNTs-AChE biosensor presented easy preparation, fast response, sensitivity, durability, good repeatability, and reproducibility.
American Psychological Association (APA)
Oliveira, A. C.& Mascaro, Lucia H.. 2011. Evaluation of Acetylcholinesterase Biosensor Based on Carbon Nanotube Paste in the Determination of Chlorphenvinphos. International Journal of Analytical Chemistry،Vol. 2011, no. 2011, pp.1-6.
https://search.emarefa.net/detail/BIM-512749
Modern Language Association (MLA)
Oliveira, A. C.& Mascaro, Lucia H.. Evaluation of Acetylcholinesterase Biosensor Based on Carbon Nanotube Paste in the Determination of Chlorphenvinphos. International Journal of Analytical Chemistry No. 2011 (2011), pp.1-6.
https://search.emarefa.net/detail/BIM-512749
American Medical Association (AMA)
Oliveira, A. C.& Mascaro, Lucia H.. Evaluation of Acetylcholinesterase Biosensor Based on Carbon Nanotube Paste in the Determination of Chlorphenvinphos. International Journal of Analytical Chemistry. 2011. Vol. 2011, no. 2011, pp.1-6.
https://search.emarefa.net/detail/BIM-512749
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-512749