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Retinal Pigment Epithelial Cells Express a Functional Receptor for Glucagon-Like Peptide-1 (GLP-1)
Joint Authors
Montecucco, Fabrizio
Puddu, Alessandra
Sanguineti, Roberta
Viviani, Giorgio Luciano
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-11-06
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Glucagon-like peptide-1 (GLP-1) is a gut-derived incretin hormone that has been shown to improve glucose homeostasis in type 2 diabetes.
The biological effects of GLP-1 are mediated by its specific receptor GLP-1R that is expressed in a wide range of tissues, where it is responsible of the extra-pancreatic effects of GLP-1.
Since the retinal pigment epithelium (RPE), that forms the outer retinal barrier, has a key role in protecting from diabetic retinopathy (DR), we investigated the potential expression and function of GLP-1R in a RPE cell line.
ARPE-19 cells were cultured in DMEM/F12 supplemented with 10% FBS.
The expression of GLP-1R was evaluated at both mRNA and protein levels.
Then, the activation postreceptor intracellular signal transduction pathways (extracellular signal-regulated kinases 1 and 2 [ERK1/2] and protein kinase B [PKB]) were assessed by western blot in normal cells or silenced for GLP-1R in the presence or absence of 10 nmol/L GLP-1.
The potential connections between intracellular signalling pathways triggered by GLP-1 stimulation were performed before incubating cells with kinase pharmacological inhibitors of mitogen-activated protein kinase (MEK)1/2, phosphatydilinositol-3kinase (PI3K), or epidermal growth factor receptor (EGFR).
The results showed that GLP1R is expressed at both mRNA and protein level in ARPE-19 cells.
Stimulation with GLP-1 strongly activated PKB and ERK1/2 phosphorylation till 40 min of exposure.
GLP-1-mediated activation of both kinases was dependent on the upstream activation of PI3K and EGFR.
Finally, treatment with GLP-1 did not affect the spontaneous release of VEGF-A from ARPE-19 cells.
In conclusion, this paper showed that the presence of functional GLP-1R is expressed in RPE cells.
These data might represent the rationale to further investigate the potential direct beneficial effects of GLP-1 treatment against DR.
American Psychological Association (APA)
Puddu, Alessandra& Sanguineti, Roberta& Montecucco, Fabrizio& Viviani, Giorgio Luciano. 2013. Retinal Pigment Epithelial Cells Express a Functional Receptor for Glucagon-Like Peptide-1 (GLP-1). Mediators of Inflammation،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-512811
Modern Language Association (MLA)
Puddu, Alessandra…[et al.]. Retinal Pigment Epithelial Cells Express a Functional Receptor for Glucagon-Like Peptide-1 (GLP-1). Mediators of Inflammation No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-512811
American Medical Association (AMA)
Puddu, Alessandra& Sanguineti, Roberta& Montecucco, Fabrizio& Viviani, Giorgio Luciano. Retinal Pigment Epithelial Cells Express a Functional Receptor for Glucagon-Like Peptide-1 (GLP-1). Mediators of Inflammation. 2013. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-512811
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-512811