Chlorotoxin Fused to IgG-Fc Inhibits Glioblastoma Cell Motility via Receptor-Mediated Endocytosis
Joint Authors
Murakami, Hiroshi
El-Ghlban, Samah
Vaidyanath, Arun
Sekhar, Sreeja
Seno, Masaharu
Kasai, Tomonari
Mizutani, Akifumi
Nakamura, Keisuke
Kudoh, Takayuki
Okada, Masashi
Chen, Ling
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-12-05
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Chlorotoxin is a 36-amino acid peptide derived from Leiurus quinquestriatus (scorpion) venom, which has been shown to inhibit low-conductance chloride channels in colonic epithelial cells.
Chlorotoxin also binds to matrix metalloproteinase-2 and other proteins on glioma cell surfaces.
Glioma cells are considered to require the activation of matrix metalloproteinase-2 during invasion and migration.
In this study, for targeting glioma, we designed two types of recombinant chlorotoxin fused to human IgG-Fcs with/without a hinge region.
Chlorotoxin fused to IgG-Fcs was designed as a dimer of 60 kDa with a hinge region and a monomer of 30 kDa without a hinge region.
The monomeric and dimeric forms of chlorotoxin inhibited cell proliferation at 300 nM and induced internalization in human glioma A172 cells.
The monomer had a greater inhibitory effect than the dimer; therefore, monomeric chlorotoxin fused to IgG-Fc was multivalently displayed on the surface of bionanocapsules to develop a drug delivery system that targeted matrix metalloproteinase-2.
The target-dependent internalization of bionanocapsules in A172 cells was observed when chlorotoxin was displayed on the bionanocapsules.
This study indicates that chlorotoxin fused to IgG-Fcs could be useful for the active targeting of glioblastoma cells.
American Psychological Association (APA)
Kasai, Tomonari& Nakamura, Keisuke& Vaidyanath, Arun& Chen, Ling& Sekhar, Sreeja& El-Ghlban, Samah…[et al.]. 2012. Chlorotoxin Fused to IgG-Fc Inhibits Glioblastoma Cell Motility via Receptor-Mediated Endocytosis. Journal of Drug Delivery،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-512871
Modern Language Association (MLA)
Kasai, Tomonari…[et al.]. Chlorotoxin Fused to IgG-Fc Inhibits Glioblastoma Cell Motility via Receptor-Mediated Endocytosis. Journal of Drug Delivery No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-512871
American Medical Association (AMA)
Kasai, Tomonari& Nakamura, Keisuke& Vaidyanath, Arun& Chen, Ling& Sekhar, Sreeja& El-Ghlban, Samah…[et al.]. Chlorotoxin Fused to IgG-Fc Inhibits Glioblastoma Cell Motility via Receptor-Mediated Endocytosis. Journal of Drug Delivery. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-512871
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-512871