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Analysis of Digoxin and Metildigoxin in Whole Blood Using Solid-Phase Extraction and Liquid Chromatography Tandem Mass Spectrometry
Joint Authors
Teixeira, Helena M.
Machado, Rita
Melo, Paula
Source
International Journal of Analytical Chemistry
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-06-22
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
A simple and rapid UPLC/MS/MS method has been developed and validated for the analysis of digoxin and metildigoxin in whole blood.
Samples were prepared by SPE extraction with Oasis HLB columns.
Separation was achieved with an ACQUITY UPLC HSS T3 column (2.1×100; 1.8 μm), at 35°C.
The mobile phase consisted of acetonitrile (70%) and ammonium formate 5 mM (30%).
Total run time was 1.5 min operating at isocratic mode with a flow rate of 0.3 mL/min.
Mass spectrometry detection was performed by positive mode electrospray, on the ammonium adducts with two transitions for each analyte and one for the IS (d3-digoxin).
The method proved to be specific and linear over the range (0.3–10) ng/mL.
This technique also showed high sensitivity with a 0.09 ng/mL LOD and a 0.28 ng/mL LOQ, for both substances.
Percentage recovery ranged from 83 to 100% for digoxin and from 62 to 94% for metildigoxin.
The intra- and interday precision CV were ≤10%.
American Psychological Association (APA)
Melo, Paula& Machado, Rita& Teixeira, Helena M.. 2011. Analysis of Digoxin and Metildigoxin in Whole Blood Using Solid-Phase Extraction and Liquid Chromatography Tandem Mass Spectrometry. International Journal of Analytical Chemistry،Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-512880
Modern Language Association (MLA)
Melo, Paula…[et al.]. Analysis of Digoxin and Metildigoxin in Whole Blood Using Solid-Phase Extraction and Liquid Chromatography Tandem Mass Spectrometry. International Journal of Analytical Chemistry No. 2012 (2012), pp.1-7.
https://search.emarefa.net/detail/BIM-512880
American Medical Association (AMA)
Melo, Paula& Machado, Rita& Teixeira, Helena M.. Analysis of Digoxin and Metildigoxin in Whole Blood Using Solid-Phase Extraction and Liquid Chromatography Tandem Mass Spectrometry. International Journal of Analytical Chemistry. 2011. Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-512880
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-512880