Chemical Composition and Anti-Inflammatory Effect of Ethanolic Extract of Brazilian Green Propolis on Activated J774A.1 Macrophages
Joint Authors
Mertas, Anna
Kucharska, Alicja Z.
Sokół-Łętowska, Anna
Szliszka, Ewelina
Czuba, Zenon P.
Krol, Wojciech
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-06-06
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
The aim of this study was to investigate the chemical composition and anti-inflammatory effect of ethanolic extract of Brazilian green propolis (EEP-B) on LPS + IFN-γ or PMA stimulated J774A.1 macrophages.
The identification and quantification of phenolic compounds in green propolis extract were performed using HPLC-DAD and UPLC-Q-TOF-MS methods.
The cell viability was evaluated by MTT and LDH assays.
The radical scavenging ability was determined using DPPH• and ABTS•+.
ROS and RNS generation was analyzed by chemiluminescence.
NO concentration was detected by the Griess reaction.
The release of various cytokines by activated J774A.1 cells was measured in the culture supernatants using a multiplex bead array system based on xMAP technology.
Artepillin C, kaempferide, and their derivatives were the main phenolics found in green propolis.
At the tested concentrations, the EEP-B did not decrease the cell viability and did not cause the cytotoxicity.
EEP-B exerted strong antioxidant activity and significantly inhibited the production of ROS, RNS, NO, cytokine IL-1α, IL-1β, IL-4, IL-6, IL-12p40, IL-13, TNF-α, G-CSF, GM-CSF, MCP-1, MIP-1α, MIP-1β, and RANTES in stimulated J774A.1 macrophages.
Our findings provide new insights for understanding the anti-inflammatory mechanism of action of Brazilian green propolis extract and support its application in complementary and alternative medicine.
American Psychological Association (APA)
Szliszka, Ewelina& Kucharska, Alicja Z.& Sokół-Łętowska, Anna& Mertas, Anna& Czuba, Zenon P.& Krol, Wojciech. 2013. Chemical Composition and Anti-Inflammatory Effect of Ethanolic Extract of Brazilian Green Propolis on Activated J774A.1 Macrophages. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-512945
Modern Language Association (MLA)
Szliszka, Ewelina…[et al.]. Chemical Composition and Anti-Inflammatory Effect of Ethanolic Extract of Brazilian Green Propolis on Activated J774A.1 Macrophages. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-13.
https://search.emarefa.net/detail/BIM-512945
American Medical Association (AMA)
Szliszka, Ewelina& Kucharska, Alicja Z.& Sokół-Łętowska, Anna& Mertas, Anna& Czuba, Zenon P.& Krol, Wojciech. Chemical Composition and Anti-Inflammatory Effect of Ethanolic Extract of Brazilian Green Propolis on Activated J774A.1 Macrophages. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-512945
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-512945