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Lipid Droplet Accumulation and Impaired Fat Efflux in Polarized Hepatic Cells : Consequences of Ethanol Metabolism
Joint Authors
Rasineni, Karuna
Casey, Carol A.
McNiven, Mark A.
Tuma, Dean J.
McVicker, Benita L.
Source
International Journal of Hepatology
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-03-11
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Steatosis, an early manifestation in alcoholic liver disease, is associated with the accumulation of hepatocellular lipid droplets (LDs).
However, the role ethanol metabolism has in LD formation and turnover remains undefined.
Here, we assessed LD dynamics following ethanol and oleic acid treatment to ethanol-metabolizing WIF-B cells (a hybrid of human fibroblasts (WI 38) and Fao rat hepatoma cells).
An OA dose-dependent increase in triglyceride and stained lipids was identified which doubled (P<0.05) in the presence of ethanol.
This effect was blunted with the inclusion of an alcohol metabolism inhibitor.
The ethanol/ OA combination also induced adipophilin, LD coat protein involved in the attenuation of lipolysis.
Additionally, ethanol treatment resulted in a significant reduction in lipid efflux.
These data demonstrate that the metabolism of ethanol in hepatic cells is related to LD accumulation, impaired fat efflux, and enhancements in LD-associated proteins.
These alterations in LD dynamics may contribute to ethanol-mediated defects in hepatocellular LD regulation and the formation of steatosis.
American Psychological Association (APA)
McVicker, Benita L.& Rasineni, Karuna& Tuma, Dean J.& McNiven, Mark A.& Casey, Carol A.. 2012. Lipid Droplet Accumulation and Impaired Fat Efflux in Polarized Hepatic Cells : Consequences of Ethanol Metabolism. International Journal of Hepatology،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-513012
Modern Language Association (MLA)
McVicker, Benita L.…[et al.]. Lipid Droplet Accumulation and Impaired Fat Efflux in Polarized Hepatic Cells : Consequences of Ethanol Metabolism. International Journal of Hepatology No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-513012
American Medical Association (AMA)
McVicker, Benita L.& Rasineni, Karuna& Tuma, Dean J.& McNiven, Mark A.& Casey, Carol A.. Lipid Droplet Accumulation and Impaired Fat Efflux in Polarized Hepatic Cells : Consequences of Ethanol Metabolism. International Journal of Hepatology. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-513012
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-513012