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Influence of Codon Bias on Heterologous Production of Human Papillomavirus Type 16 Major Structural Protein L1 in Yeast
Joint Authors
Gedvilaite, Alma
Norkiene, Milda
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-05-02
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Medicine
Information Technology and Computer Science
Abstract EN
Heterologous gene expression is dependent on multistep processes involving regulation at the level of transcription, mRNA turnover, protein translation, and posttranslational modifications.
Codon bias has a significant influence on protein yields.
However, sometimes it is not clear which parameter causes observed differences in heterologous gene expression as codon adaptation typically optimizes many sequence properties at once.
In the current study, we evaluated the influence of codon bias on heterologous production of human papillomavirus type 16 (HPV-16) major structural protein L1 in yeast by expressing five variants of codon-modified open reading frames (OFRs) encoding HPV-16 L1 protein.
Our results showed that despite the high toleration of various codons used throughout the length of the sequence of heterologously expressed genes in transformed yeast, there was a significant positive correlation between the gene's expression level and the degree of its codon bias towards the favorable codon usage.
The HPV-16 L1 protein expression in yeast can be optimized by adjusting codon composition towards the most preferred codon adaptation, and this effect most probably is dependent on the improved translational elongation.
American Psychological Association (APA)
Norkiene, Milda& Gedvilaite, Alma. 2012. Influence of Codon Bias on Heterologous Production of Human Papillomavirus Type 16 Major Structural Protein L1 in Yeast. The Scientific World Journal،Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-513108
Modern Language Association (MLA)
Norkiene, Milda& Gedvilaite, Alma. Influence of Codon Bias on Heterologous Production of Human Papillomavirus Type 16 Major Structural Protein L1 in Yeast. The Scientific World Journal No. 2012 (2012), pp.1-6.
https://search.emarefa.net/detail/BIM-513108
American Medical Association (AMA)
Norkiene, Milda& Gedvilaite, Alma. Influence of Codon Bias on Heterologous Production of Human Papillomavirus Type 16 Major Structural Protein L1 in Yeast. The Scientific World Journal. 2012. Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-513108
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-513108