Fine Structure of Glycosaminoglycans from Fresh and Decellularized Porcine Cardiac Valves and Pericardium
Joint Authors
Gastaldello, Alessandra
Naso, Filippo
Gandaglia, Alessandro
Formato, Marilena
Cigliano, Antonio
Aguiari, Paola
Zinellu, Elisabetta
Spina, Michele
De Muro, Pierina
Gerosa, Gino
Lepedda, Antonio Junior
Source
Biochemistry Research International
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-02-22
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Cardiac valves are dynamic structures, exhibiting a highly specialized architecture consisting of cells and extracellular matrix with a relevant proteoglycan and glycosaminoglycan content, collagen and elastic fibers.
Biological valve substitutes are obtained from xenogenic cardiac and pericardial tissues.
To overcome the limits of such non viable substitutes, tissue engineering approaches emerged to create cell repopulated decellularized scaffolds.
This study was performed to determine the glycosaminoglycans content, distribution, and disaccharides composition in porcine aortic and pulmonary valves and in pericardium before and after a detergent-based decellularization procedure.
The fine structural characteristics of galactosaminoglycans chondroitin sulfate and dermatan sulfate were examined by FACE.
Furthermore, the mechanical properties of decellularized pericardium and its propensity to be repopulated by in vitro seeded fibroblasts were investigated.
Results show that galactosaminoglycans and hyaluronan are differently distributed between pericardium and valves and within heart valves themselves before and after decellularization.
The distribution of glycosaminoglycans is also dependent from the vascular district and topographic localization.
The decellularization protocol adopted resulted in a relevant but not selective depletion of galactosaminoglycans.
As a whole, data suggest that both decellularized porcine heart valves and bovine pericardium represent promising materials bearing the potential for future development of tissue engineered heart valve scaffolds.
American Psychological Association (APA)
Cigliano, Antonio& Gandaglia, Alessandro& Lepedda, Antonio Junior& Zinellu, Elisabetta& Naso, Filippo& Gastaldello, Alessandra…[et al.]. 2012. Fine Structure of Glycosaminoglycans from Fresh and Decellularized Porcine Cardiac Valves and Pericardium. Biochemistry Research International،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-513116
Modern Language Association (MLA)
Cigliano, Antonio…[et al.]. Fine Structure of Glycosaminoglycans from Fresh and Decellularized Porcine Cardiac Valves and Pericardium. Biochemistry Research International No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-513116
American Medical Association (AMA)
Cigliano, Antonio& Gandaglia, Alessandro& Lepedda, Antonio Junior& Zinellu, Elisabetta& Naso, Filippo& Gastaldello, Alessandra…[et al.]. Fine Structure of Glycosaminoglycans from Fresh and Decellularized Porcine Cardiac Valves and Pericardium. Biochemistry Research International. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-513116
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-513116