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Paraoxonase and Arylesterase Activities, Lipid Profile, and Oxidative Damage in Experimental Ischemic Colitis Model
Joint Authors
Kutanis, Riza
Yildar, Murat
Eris, Cengiz
Kiziler, Ali Riza
Unal, Ethem
Kaya, Bülent
Cavdar, Faruk
Titiz, Izzet
Uzun, Hafize
Gunes, Pembegul
Aydemir, Birsen
Source
Gastroenterology Research and Practice
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-11-12
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
Objective.
In the present study, since PON1 is known as an HDL-associated antioxidant enzyme that inhibits the oxidative modification of LDL and oxidative stress plays a role in the pathogenesis of mesenteric ischemia, we investigated the changes in PON1 activity and lipid profile in an experimental ischemic colitis model.
Methods.
Forty male Wistar albino rats were divided into two groups: the control group (N=15) and the experimental group (N=25).
All animals were anesthetized with ether and ketamine anesthesia to undergo a midline laparotomy.
Ischemic colitis was induced by marginal vessel ligation in the splenic flexura (devascularization process).
A sham laparotomy was performed in the control group.
All animals were sacrificed on the seventh postoperative day.
Oxidative stress marker (malonyldialdehyde, MDA), lipid profile, and paraoxonase (PON-1) and arylesterase activities were determined.
Histopathological evaluation was done under light microscopy, after sectioning and staining with hematoxyline and eosin.
Statistical analysis was conducted using Student’s t-test and Mann-Whitney U test, and P<0.05 was considered as statistically significant.
Results.
There was a significant decrease in both serum and tissue PON1 activity in ischemic colitis group (P<0.01, for each).
Similarly, arylesterase levels showed a parallel decrease in both tissue and serum of the experimental group (P<0.01 and P<0.001, retrospectively).
MDA, an oxidative stress marker, was seen to increase in the experimental group (P<0.01, tissue; P<0.05, serum).
In experimental group, there was a significant rise in serum total cholesterol and LDL levels (P<0.001, for each).
However, HDL level decreased significantly (P<0.001).
Triglycerides did not show any change between the groups (P>0.05).
Conclusions.
PON1 and arylesterase play an important role in the pathophysiology of ischemic colitis.
American Psychological Association (APA)
Unal, Ethem& Eris, Cengiz& Kaya, Bülent& Uzun, Hafize& Cavdar, Faruk& Yildar, Murat…[et al.]. 2012. Paraoxonase and Arylesterase Activities, Lipid Profile, and Oxidative Damage in Experimental Ischemic Colitis Model. Gastroenterology Research and Practice،Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-513124
Modern Language Association (MLA)
Unal, Ethem…[et al.]. Paraoxonase and Arylesterase Activities, Lipid Profile, and Oxidative Damage in Experimental Ischemic Colitis Model. Gastroenterology Research and Practice No. 2012 (2012), pp.1-5.
https://search.emarefa.net/detail/BIM-513124
American Medical Association (AMA)
Unal, Ethem& Eris, Cengiz& Kaya, Bülent& Uzun, Hafize& Cavdar, Faruk& Yildar, Murat…[et al.]. Paraoxonase and Arylesterase Activities, Lipid Profile, and Oxidative Damage in Experimental Ischemic Colitis Model. Gastroenterology Research and Practice. 2012. Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-513124
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-513124