Molecular Genetic Diversity and Quantitation of Methanogen in Ruminal Fluid of Buffalo (Bubalus bubalis) Fed Ration (Wheat Straw and Concentrate Mixture Diet)
Joint Authors
Pandya, P. R.
Parnerkar, S.
Joshi, C. G.
Singh, K. M.
Kothari, R. K.
Tripathi, A. K.
Source
Genetics Research International
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-06-05
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
High roughage diet causes more methane emissions; however, the total methanogen abundance is not influenced by roughage proportion.
Technologies to reduce methane emissions are lacking, and development of inhibitors and vaccines that mitigate rumen-derived methane by targeting methanogens relies on present knowledge of the methanogens.
In this work, we have investigated molecular diversity of rumen methanogens of Surti buffalo.
DNA from rumen fluid was extracted, and 16S rRNA encoding genes were amplified using methanogen specific primer to generate 16S rDNA clone libraries.
Seventy-six clones were randomly selected and analysed by RFLP resulting in 21 operational taxonomic units (OTUs).
BLAST analysis with available sequences in database revealed sequences of 13 OTUs (55 clones) showing similarity with Methanomicrobium sp, 3 OTUs (15 clones) with Methanobrevibacter sp.
The remaining 5 OTUs (6 clones) belonged to uncultured archaea.
The phylogenetic analysis indicated that methanogenic communities found in the library were clustered in the order of Methanomicrobiales (18 OTUs) and Methanobacteriales (3 OTUs).
The population of Methanomicrobiales, Methanobacteriales, and Methanococcales were also observed, accounting for 1.94%, 0.72%, and 0.47% of total archaea, respectively.
American Psychological Association (APA)
Singh, K. M.& Tripathi, A. K.& Pandya, P. R.& Parnerkar, S.& Kothari, R. K.& Joshi, C. G.. 2013. Molecular Genetic Diversity and Quantitation of Methanogen in Ruminal Fluid of Buffalo (Bubalus bubalis) Fed Ration (Wheat Straw and Concentrate Mixture Diet). Genetics Research International،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-513170
Modern Language Association (MLA)
Singh, K. M.…[et al.]. Molecular Genetic Diversity and Quantitation of Methanogen in Ruminal Fluid of Buffalo (Bubalus bubalis) Fed Ration (Wheat Straw and Concentrate Mixture Diet). Genetics Research International No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-513170
American Medical Association (AMA)
Singh, K. M.& Tripathi, A. K.& Pandya, P. R.& Parnerkar, S.& Kothari, R. K.& Joshi, C. G.. Molecular Genetic Diversity and Quantitation of Methanogen in Ruminal Fluid of Buffalo (Bubalus bubalis) Fed Ration (Wheat Straw and Concentrate Mixture Diet). Genetics Research International. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-513170
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-513170