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Constitutive High Level Expression of an Endoxylanase Gene from the Newly Isolated Bacillus subtilis AQ1 in Escherichia coli
Joint Authors
Ulfah, Maria
Setyahadi, Siswa
Wahyuntari, Budiasih
Nurhayati, Niknik
Helianti, Is
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-09-21
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
A xylanolytic bacterium was isolated from the sediment of an aquarium.
Based on the 16S rDNA sequence as well as morphological and biochemical properties the isolate was identified and denoted as Bacillus subtilis (B.
subtilis) AQ1 strain.
An endoxylanase-encoding gene along with its indigenous promoter was PCR amplified and after cloning expressed in E.
coli.
In E.
coli the recombinant enzyme was found in the extracellular, in the cytoplasmic, and in the periplasmic fraction.
The specific activity of the extracellular AQ1 recombinant endoxylanase after 24-hour fermentation was very high, namely, 2173.6 ± 51.4 and 2745.3 ± 11 U/mg in LB and LB-xylan medium, respectively.
This activity was clearly exceeding that of the native B.
subtilis AQ1 endoxylanase and that of 95% homologous recombinant one from B.
subtilis DB104.
The result shows that the original AQ1 endoxylanase promoter and the signal peptide gave a very high constitutive extracellular expression in E.
coli and hence made the production in E.
coli feasible.
American Psychological Association (APA)
Helianti, Is& Nurhayati, Niknik& Ulfah, Maria& Wahyuntari, Budiasih& Setyahadi, Siswa. 2010. Constitutive High Level Expression of an Endoxylanase Gene from the Newly Isolated Bacillus subtilis AQ1 in Escherichia coli. Journal of Biomedicine and Biotechnology،Vol. 2010, no. 2010, pp.1-12.
https://search.emarefa.net/detail/BIM-513205
Modern Language Association (MLA)
Helianti, Is…[et al.]. Constitutive High Level Expression of an Endoxylanase Gene from the Newly Isolated Bacillus subtilis AQ1 in Escherichia coli. Journal of Biomedicine and Biotechnology No. 2010 (2010), pp.1-12.
https://search.emarefa.net/detail/BIM-513205
American Medical Association (AMA)
Helianti, Is& Nurhayati, Niknik& Ulfah, Maria& Wahyuntari, Budiasih& Setyahadi, Siswa. Constitutive High Level Expression of an Endoxylanase Gene from the Newly Isolated Bacillus subtilis AQ1 in Escherichia coli. Journal of Biomedicine and Biotechnology. 2010. Vol. 2010, no. 2010, pp.1-12.
https://search.emarefa.net/detail/BIM-513205
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-513205