An Amperometric Biosensor Based on Alanine Dehydrogenase for the Determination of Low Level of Ammonium Ion in Water
Joint Authors
Heng, Lee Yook
Ahmad, Musa
Ling Ling, Tan
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-09-06
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
An amperometric electrochemical biosensor has been developed for ammonium (NH4+) ion detection by immobilising alanine dehydrogenase (AlaDH) enzyme in a photocurable methacrylic membrane made up of poly(2-hydroxyethyl methacrylate) (pHEMA) on a screen-printed carbon paste electrode (SPE).
The current detected was based on the electrocatalytic oxidation of nicotinamide adenine dinucleotide reduced (NADH) that is proportional to the consumption of NH4+ ion whilst enzymatic amination of AlaDH and pyruvate is taking place.
The biosensor was operated amperometrically at a potential of +0.6 V and optimum pH 7.
The NH4+ biosensor demonstrated linear response to NH4+ ion concentration in the range of 0.03–1.02 mg/L with a limit of detection (LOD) of 8.52 μg/L.
The proposed method has been successfully applied to the determination of NH4+ ion in river water samples without any pretreatment.
The levels of possible interferents in the waters were negligible to cause any interference on the proposed method.
The analytical performance of the biosensor was comparable to the colorimetric method using Nesslerisation but with much lower detection limit and linear response range at ppb level.
American Psychological Association (APA)
Ling Ling, Tan& Ahmad, Musa& Heng, Lee Yook. 2011. An Amperometric Biosensor Based on Alanine Dehydrogenase for the Determination of Low Level of Ammonium Ion in Water. Journal of Sensors،Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-513223
Modern Language Association (MLA)
Ling Ling, Tan…[et al.]. An Amperometric Biosensor Based on Alanine Dehydrogenase for the Determination of Low Level of Ammonium Ion in Water. Journal of Sensors No. 2011 (2011), pp.1-10.
https://search.emarefa.net/detail/BIM-513223
American Medical Association (AMA)
Ling Ling, Tan& Ahmad, Musa& Heng, Lee Yook. An Amperometric Biosensor Based on Alanine Dehydrogenase for the Determination of Low Level of Ammonium Ion in Water. Journal of Sensors. 2011. Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-513223
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-513223