Gene Expression Changes in Venous Segment of Overflow Arteriovenous Fistula
Joint Authors
Okamoto, Akiko
Ohyama, Chikara
Hashimoto, Yasuhiro
Koie, Takuya
Saitoh, Hisao
Hatakeyama, Shingo
Yoneyama, Takahiro
Source
International Journal of Nephrology
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-04-27
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Aim.
The objective of this study was to characterize coordinated molecular changes in the structure and composition of the walls of venous segments of arteriovenous (AV) fistulas evoked by overflow.
Methods.
Venous tissue samples were collected from 6 hemodialysis patients with AV fistulas exposed to overflow and from the normal cephalic veins of 4 other hemodialysis patients.
Total RNA was extracted from the venous tissue samples, and gene expression between the 2 groups was compared using Whole Human Genome DNA microarray 44 K.
Microarray data were analyzed by GeneSpring GX software and Ingenuity Pathway Analysis.
Results.
The cDNA microarray analysis identified 397 upregulated genes and 456 downregulated genes.
Gene ontology analysis with GeneSpring GX software revealed that biological developmental processes and glycosaminoglycan binding were the most upregulated.
In addition, most upregulation occurred extracellularly.
In the pathway analysis, the TGF beta signaling pathway, cytokines and inflammatory response pathway, hypertrophy model, and the myometrial relaxation and contraction pathway were significantly upregulated compared with the control cephalic vein.
Conclusion.
Combining microarray results and pathway information available via the Internet provided biological insight into the structure and composition of the venous wall of overflow AV fistulas.
American Psychological Association (APA)
Hashimoto, Yasuhiro& Okamoto, Akiko& Saitoh, Hisao& Hatakeyama, Shingo& Yoneyama, Takahiro& Koie, Takuya…[et al.]. 2013. Gene Expression Changes in Venous Segment of Overflow Arteriovenous Fistula. International Journal of Nephrology،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-513249
Modern Language Association (MLA)
Hashimoto, Yasuhiro…[et al.]. Gene Expression Changes in Venous Segment of Overflow Arteriovenous Fistula. International Journal of Nephrology No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-513249
American Medical Association (AMA)
Hashimoto, Yasuhiro& Okamoto, Akiko& Saitoh, Hisao& Hatakeyama, Shingo& Yoneyama, Takahiro& Koie, Takuya…[et al.]. Gene Expression Changes in Venous Segment of Overflow Arteriovenous Fistula. International Journal of Nephrology. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-513249
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-513249