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Genome-Wide Analysis of Gene and Protein Expression of Dysplastic Naevus Cells
Joint Authors
Out-Luiting, Jacoba J.
Gruis, Nelleke A.
Bergman, Wilma
van Nieuwpoort, Frans A.
Gao, Linda
Hensbergen, Paul J.
de Snoo, Femke A.
van Doorn, Remco
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-11-28
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Cutaneous melanoma, a type of skin tumor originating from melanocytes, often develops from premalignant naevoid lesions via a gradual transformation process driven by an accumulation of (epi)genetic lesions.
These dysplastic naevi display altered morphology and often proliferation of melanocytes.
Additionally, melanocytes in dysplastic naevi show structural mitochondrial and melanosomal alterations and have elevated reactive oxygen species (ROS) levels.
For this study we performed genome-wide expression and proteomic analysis of melanocytes from dysplastic naevus (DNMC) and adjacent normal skin (MC) from 18 patients.
Whole genome expression profiles of the DNMC and MC of each individual patient subjected to GO-based comparative statistical analysis yielded significantly differentially expressed GO classes including “organellar ribosome,” “mitochondrial ribosome,” “hydrogen ion transporter activity,” and “prefoldin complex.” Validation of 5 genes from these top GO classes revealed a heterogeneous differential expression pattern.
Proteomic analysis demonstrated differentially expressed proteins in DNMC that are involved in cellular metabolism, detoxification, and cytoskeletal organization processes, such as GTP-binding Rho-like protein CDC42, glutathione-S-transferase omega-1 and prolyl 4-hydroxylase.
Collectively these results point to deregulation of cellular processes, such as metabolism and protein synthesis, consistent with the observed elevated oxidative stress levels in DNMC potentially resulting in oxidative DNA damage in these cells.
American Psychological Association (APA)
Gao, Linda& van Nieuwpoort, Frans A.& Out-Luiting, Jacoba J.& Hensbergen, Paul J.& de Snoo, Femke A.& Bergman, Wilma…[et al.]. 2012. Genome-Wide Analysis of Gene and Protein Expression of Dysplastic Naevus Cells. Journal of Skin Cancer،Vol. 2012, no. 2012, pp.1-13.
https://search.emarefa.net/detail/BIM-513284
Modern Language Association (MLA)
Gao, Linda…[et al.]. Genome-Wide Analysis of Gene and Protein Expression of Dysplastic Naevus Cells. Journal of Skin Cancer No. 2012 (2012), pp.1-13.
https://search.emarefa.net/detail/BIM-513284
American Medical Association (AMA)
Gao, Linda& van Nieuwpoort, Frans A.& Out-Luiting, Jacoba J.& Hensbergen, Paul J.& de Snoo, Femke A.& Bergman, Wilma…[et al.]. Genome-Wide Analysis of Gene and Protein Expression of Dysplastic Naevus Cells. Journal of Skin Cancer. 2012. Vol. 2012, no. 2012, pp.1-13.
https://search.emarefa.net/detail/BIM-513284
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-513284