Covalent Attachment of Carbohydrate Derivatives to an Evanescent Wave Fiber Bragg Grating Biosensor

Abstract EN

A carbohydrate-based biosensor was prepared by functionalization of the surface of an etched fiber Bragg grating with a glucopyranosyl-siloxane conjugate.

Functionalization of the surface with the conjugate resulted in a Bragg grating shift of 24 pm.

This shift in the refractive index is consistent with a theoretical shift calculated assuming monolayer coverage of the glucose conjugate on the sensor.

The resulting functionalized fiber was shown to interact selectively with concanavalin A (Con A), a glucose binding protein (lectin).

Exposure of the glucose-functionalized fiber to peanut agglutinin, a galactosebinding lectin, did not result in a change of the refractive index corresponding to a binding event.