Holocarboxylase Synthetase 1 Physically Interacts with Histone H3 in Arabidopsis
Joint Authors
Wurtele, Eve Syrkin
Chen, Xi
Chou, Hui-Hsien
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-02-12
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Diseases
Abstract EN
Biotin is a water-soluble vitamin required by all organisms, but only synthesized by plants and some bacterial and fungal species.
As a cofactor, biotin is responsible for carbon dioxide transfer in all biotin-dependent carboxylases, including acetyl-CoA carboxylase, methylcrotonyl-CoA carboxylase, and pyruvate carboxylase.
Adding biotin to carboxylases is catalyzed by the enzyme holocarboxylase synthetase (HCS).
Biotin is also involved in gene regulation, and there is some indication that histones can be biotinylated in humans.
Histone proteins and most histone modifications are highly conserved among eukaryotes.
HCS1 is the only functional biotin ligase in Arabidopsis and has a high homology with human HCS.
Therefore, we hypothesized that HCS1 also biotinylates histone proteins in Arabidopsis.
A comparison of the catalytic domain of HCS proteins was performed among eukaryotes, prokaryotes, and archaea, and this domain is highly conserved across the selected organisms.
Biotinylated histones could not be identified in vivo by using avidin precipitation or two-dimensional gel analysis.
However, HCS1 physically interacts with Arabidopsis histone H3 in vitro, indicating the possibility of the role of this enzyme in the regulation of gene expression.
American Psychological Association (APA)
Chen, Xi& Chou, Hui-Hsien& Wurtele, Eve Syrkin. 2013. Holocarboxylase Synthetase 1 Physically Interacts with Histone H3 in Arabidopsis. Scientifica،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-513491
Modern Language Association (MLA)
Chen, Xi…[et al.]. Holocarboxylase Synthetase 1 Physically Interacts with Histone H3 in Arabidopsis. Scientifica No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-513491
American Medical Association (AMA)
Chen, Xi& Chou, Hui-Hsien& Wurtele, Eve Syrkin. Holocarboxylase Synthetase 1 Physically Interacts with Histone H3 in Arabidopsis. Scientifica. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-513491
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-513491