Epitope Mapping of Metuximab on CD147 Using Phage Display and Molecular Docking
Joint Authors
Zhou, Peng
Huang, Jian
Mao, Canquan
Ru, Beibei
He, Bifang
Han, Hesong
Source
Computational and Mathematical Methods in Medicine
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-06-03
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Metuximab is the generic name of Licartin, a new drug for radioimmunotherapy of hepatocellular carcinoma.
Although it is known to be a mouse monoclonal antibody against CD147, the complete epitope mediating the binding of metuximab to CD147 remains unknown.
We panned the Ph.D.-12 phage display peptide library against metuximab and got six mimotopes.
The following bioinformatics analysis based on mimotopes suggested that metuximab recognizes a conformational epitope composed of more than 20 residues.
The residues of its epitope may include T28, V30, K36, L38, K57, F74, D77, S78, D79, D80, Q81, G83, S86, N98, Q100, L101, H102, G103, P104, V131, P132, and K191.
The homology modeling of metuximab and the docking of CD147 to metuximab were also performed.
Based on the top one docking model, the epitope was predicted to contain 28 residues: AGTVFTTV (23–30), I37, D45, E84, V88, EPMGTANIQLH (92–102), VPP (131–133), Q164, and K191.
Almost half of the residues predicted on the basis of mimotope analysis also appear in the docking result, indicating that both results are reliable.
As the predicted epitopes of metuximab largely overlap with interfaces of CD147-CD147 interactions, a structural mechanism of metuximab is proposed as blocking the formation of CD147 dimer.
American Psychological Association (APA)
He, Bifang& Mao, Canquan& Ru, Beibei& Han, Hesong& Zhou, Peng& Huang, Jian. 2013. Epitope Mapping of Metuximab on CD147 Using Phage Display and Molecular Docking. Computational and Mathematical Methods in Medicine،Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-513526
Modern Language Association (MLA)
He, Bifang…[et al.]. Epitope Mapping of Metuximab on CD147 Using Phage Display and Molecular Docking. Computational and Mathematical Methods in Medicine No. 2013 (2013), pp.1-6.
https://search.emarefa.net/detail/BIM-513526
American Medical Association (AMA)
He, Bifang& Mao, Canquan& Ru, Beibei& Han, Hesong& Zhou, Peng& Huang, Jian. Epitope Mapping of Metuximab on CD147 Using Phage Display and Molecular Docking. Computational and Mathematical Methods in Medicine. 2013. Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-513526
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-513526