Identification and Characterization of Cell Wall Proteins of a Toxic Dinoflagellate Alexandrium catenella Using 2-D DIGE and MALDI TOF-TOF Mass Spectrometry
Joint Authors
Xie, Zhang-Xian
Li, Cheng
Dong, Hong-Po
Wang, Da-Zhi
Lin, Lin
Hong, Hua-Sheng
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-09-05
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
The cell wall is an important subcellular component of dinoflagellate cells with regard to various aspects of cell surface-associated ecophysiology, but the full range of cell wall proteins (CWPs) and their functions remain to be elucidated.
This study identified and characterized CWPs of a toxic dinoflagellate, Alexandrium catenella, using a combination of 2D fluorescence difference gel electrophoresis (DIGE) and MALDI TOF-TOF mass spectrometry approaches.
Using sequential extraction and temperature shock methods, sequentially extracted CWPs and protoplast proteins, respectively, were separated from A.
catenella.
From the comparison between sequentially extracted CWPs labeled with Cy3 and protoplast proteins labeled with Cy5, 120 CWPs were confidently identified in the 2D DIGE gel.
These proteins gave positive identification of protein orthologues in the protein database using de novo sequence analysis and homology-based search.
The majority of the prominent CWPs identified were hypothetical or putative proteins with unknown function or no annotation, while cell wall modification enzymes, cell wall structural proteins, transporter/binding proteins, and signaling and defense proteins were tentatively identified in agreement with the expected role of the extracellular matrix in cell physiology.
This work represents the first attempt to investigate dinoflagellate CWPs and provides a potential tool for future comprehensive characterization of dinoflagellate CWPs and elucidation of their physiological functions.
American Psychological Association (APA)
Wang, Da-Zhi& Dong, Hong-Po& Li, Cheng& Xie, Zhang-Xian& Lin, Lin& Hong, Hua-Sheng. 2011. Identification and Characterization of Cell Wall Proteins of a Toxic Dinoflagellate Alexandrium catenella Using 2-D DIGE and MALDI TOF-TOF Mass Spectrometry. Evidence-Based Complementary and Alternative Medicine،Vol. 2011, no. 2011, pp.1-11.
https://search.emarefa.net/detail/BIM-513554
Modern Language Association (MLA)
Wang, Da-Zhi…[et al.]. Identification and Characterization of Cell Wall Proteins of a Toxic Dinoflagellate Alexandrium catenella Using 2-D DIGE and MALDI TOF-TOF Mass Spectrometry. Evidence-Based Complementary and Alternative Medicine No. 2011 (2011), pp.1-11.
https://search.emarefa.net/detail/BIM-513554
American Medical Association (AMA)
Wang, Da-Zhi& Dong, Hong-Po& Li, Cheng& Xie, Zhang-Xian& Lin, Lin& Hong, Hua-Sheng. Identification and Characterization of Cell Wall Proteins of a Toxic Dinoflagellate Alexandrium catenella Using 2-D DIGE and MALDI TOF-TOF Mass Spectrometry. Evidence-Based Complementary and Alternative Medicine. 2011. Vol. 2011, no. 2011, pp.1-11.
https://search.emarefa.net/detail/BIM-513554
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-513554