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Regulation of Hypoxia-Induced Cell Death in Human Tenocytes
Joint Authors
Liang, Min
Cornell, Hannah R.
Thompson, Mark S.
Carr, Andrew J.
Zargar Baboldashti, Nasim
Hulley, Philippa A.
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-12-06
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Degenerate shoulder tendons display evidence of hypoxia.
However tendons are relatively avascular and not considered to have high oxygen requirements and the vulnerability of tendon cells to hypoxia is unclear.
Cultured human tenocytes were exposed to hypoxia and the cellular response detected using QPCR, Western blotting, viability, and ELISA assays.
We find that tenocytes respond to hypoxia in vitro by activating classical HIF-1α-driven pathways.
Total hypoxia caused significant tenocyte apoptosis.
Transcription factors typically involved in hypoxic response, HIF-1α and FOXO3A, were upregulated.
Hypoxia caused sustained upregulation of several proapoptotic proteins known to mediate hypoxia-induced apoptosis, such as Bnip3 and Nix, but others were unchanged although they were reportedly hypoxia-sensitive in other cell types.
Antiapoptotic proteins Bcl2 and Bcl-xL were unchanged by hypoxia.
Normal human tenocytes expressed all isoforms of the hypoxia-induced vascular growth factor VEGF except VEGF-D.
Hypoxia markedly upregulated VEGF-A mRNA, followed by increased VEGF protein secretion.
However treatment with VEGF did not improve tenocyte survival.
As a protective strategy for tenocytes at risk of hypoxic death we added prosurvival growth factors insulin or platelet rich plasma (PRP).
Both agents strongly protected tenocytes from hypoxia-induced death over 48 h, suggesting possible efficacy in the acute postrupture tendon or integrating graft.
American Psychological Association (APA)
Liang, Min& Cornell, Hannah R.& Zargar Baboldashti, Nasim& Thompson, Mark S.& Carr, Andrew J.& Hulley, Philippa A.. 2012. Regulation of Hypoxia-Induced Cell Death in Human Tenocytes. Advances in Orthopedics،Vol. 2012, no. 2012, pp.1-12.
https://search.emarefa.net/detail/BIM-513657
Modern Language Association (MLA)
Liang, Min…[et al.]. Regulation of Hypoxia-Induced Cell Death in Human Tenocytes. Advances in Orthopedics No. 2012 (2012), pp.1-12.
https://search.emarefa.net/detail/BIM-513657
American Medical Association (AMA)
Liang, Min& Cornell, Hannah R.& Zargar Baboldashti, Nasim& Thompson, Mark S.& Carr, Andrew J.& Hulley, Philippa A.. Regulation of Hypoxia-Induced Cell Death in Human Tenocytes. Advances in Orthopedics. 2012. Vol. 2012, no. 2012, pp.1-12.
https://search.emarefa.net/detail/BIM-513657
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-513657