Serum Peptidome Patterns of Colorectal Cancer Based on Magnetic Bead Separation and MALDI-TOF Mass Spectrometry Analysis
Joint Authors
Wang, Xiu-Li
Zhao, Guang
Liu, Qing-Yin
Gao, Chun-fang
Fan, Nai-Jun
Zhang, Yuan-Yao
Cheng, Bao-Guo
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-10-03
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Background.
Colorectal cancer (CRC) is one of the most common cancers in the world, identification of biomarkers for early detection of CRC represents a relevant target.
The present study aims to determine serum peptidome patterns for CRC diagnosis.
Methods.
The present work focused on serum proteomic analysis of 32 health volunteers and 38 CRC by ClinProt Kit combined with mass spectrometry.
This approach allowed the construction of a peptide patterns able to differentiate the studied populations.
An independent group of serum (including 33 health volunteers, 34 CRC, 16 colorectal adenoma, 36 esophageal carcinoma, and 31 gastric carcinoma samples) was used to verify the diagnostic and differential diagnostic capability of the peptidome patterns blindly.
An immunoassay method was used to determine serum CEA of CRC and controls.
Results.
A quick classifier algorithm was used to construct the peptidome patterns for identification of CRC from controls.
Two of the identified peaks at m/z 741 and 7772 were used to construct peptidome patterns, achieving an accuracy close to 100% (>CEA, P<0.05).
Furthermore, the peptidome patterns could differentiate validation group with high accuracy.
Conclusions.
These results suggest that the ClinProt Kit combined with mass spectrometry yields significantly higher accuracy for the diagnosis and differential diagnosis of CRC.
American Psychological Association (APA)
Fan, Nai-Jun& Gao, Chun-fang& Wang, Xiu-Li& Zhao, Guang& Liu, Qing-Yin& Zhang, Yuan-Yao…[et al.]. 2012. Serum Peptidome Patterns of Colorectal Cancer Based on Magnetic Bead Separation and MALDI-TOF Mass Spectrometry Analysis. Journal of Biomedicine and Biotechnology،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-513664
Modern Language Association (MLA)
Fan, Nai-Jun…[et al.]. Serum Peptidome Patterns of Colorectal Cancer Based on Magnetic Bead Separation and MALDI-TOF Mass Spectrometry Analysis. Journal of Biomedicine and Biotechnology No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-513664
American Medical Association (AMA)
Fan, Nai-Jun& Gao, Chun-fang& Wang, Xiu-Li& Zhao, Guang& Liu, Qing-Yin& Zhang, Yuan-Yao…[et al.]. Serum Peptidome Patterns of Colorectal Cancer Based on Magnetic Bead Separation and MALDI-TOF Mass Spectrometry Analysis. Journal of Biomedicine and Biotechnology. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-513664
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-513664