A Strong Impact of Genetic Background on Gut Microflora in Mice
Joint Authors
Smith, David D.
Esworthy, R. Steven
Chu, Fong-Fong
Source
International Journal of Inflammation
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-07-20
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Genetic background affects susceptibility to ileocolitis in mice deficient in two intracellular glutathione peroxidases, GPx1 and GPx2.
The C57BL/6 (B6) GPx1/2 double-knockout (DKO) mice have mild ileocolitis, and 129S1/Sv (129) DKO mice have severe inflammation.
We used diet to modulate ileocolitis; a casein-based defined diet with AIN76A micronutrients (AIN) attenuates inflammation compared to conventional LabDiets.
Because luminal microbiota induce DKO ileocolitis, we assessed bacterial composition with automated ribosomal intergenic-spacer analysis (ARISA) on cecal DNA.
We found that mouse strain had the strongest impact on the composition of microbiota than diet and GPx genotypes.
In comparing AIN and LabDiet, DKO mice were more resistant to change than the non-DKO or WT mice.
However, supplementing yeast and inulin to AIN diet greatly altered microflora profiles in the DKO mice.
From 129 DKO strictly, we found overgrowth of Escherichia coli.
We conclude that genetic background predisposes mice to colonization of potentially pathogenic E.
coli.
American Psychological Association (APA)
Esworthy, R. Steven& Smith, David D.& Chu, Fong-Fong. 2010. A Strong Impact of Genetic Background on Gut Microflora in Mice. International Journal of Inflammation،Vol. 2010, no. 2010, pp.1-12.
https://search.emarefa.net/detail/BIM-513768
Modern Language Association (MLA)
Esworthy, R. Steven…[et al.]. A Strong Impact of Genetic Background on Gut Microflora in Mice. International Journal of Inflammation No. 2010 (2010), pp.1-12.
https://search.emarefa.net/detail/BIM-513768
American Medical Association (AMA)
Esworthy, R. Steven& Smith, David D.& Chu, Fong-Fong. A Strong Impact of Genetic Background on Gut Microflora in Mice. International Journal of Inflammation. 2010. Vol. 2010, no. 2010, pp.1-12.
https://search.emarefa.net/detail/BIM-513768
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-513768