Formulation Strategies, Characterization, and In Vitro Evaluation of Lecithin-Based Nanoparticles for siRNA Delivery
Joint Authors
Turyn, Daniel
Pérez, Sebastián Ezequiel
Carlucci, Adriana Mónica
Gándola, Yamila B.
Gonzalez, Lorena
Bregni, Carlos
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-04-05
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The aim of the present work was to take advantage of lecithin’s biocompatibility along with its physicochemical properties for the preparation of lecithin-based nanocarriers for small interfering RNA (siRNA) delivery.
Water lecithin dispersions were prepared in different conditions, loaded with siRNA at different N/P ratios, and evaluated for loading capacity.
The most appropriate ones were then assayed for cytotoxicity and characterized in terms of particle size distribution, zeta potential, and morphology.
Results demonstrated that formulations prepared at pH 5.0 and 7.0 were able to load siRNA at broad N/P ratios, and cellular uptake assays showed an efficient delivery of oligos in MCF-7 human breast cancer cells; fluorescent-labeled dsRNA mainly located next to its target, near the nucleus of the cells.
No signs of toxicity were observed for broad compositions of lecithin.
The physicochemical characterization of the siRNA-loaded dispersions exhibited particles of nanometric sizes and pH-dependant shapes, which make them suitable for ex vivo and in vivo further evaluation.
American Psychological Association (APA)
Pérez, Sebastián Ezequiel& Gándola, Yamila B.& Carlucci, Adriana Mónica& Gonzalez, Lorena& Turyn, Daniel& Bregni, Carlos. 2012. Formulation Strategies, Characterization, and In Vitro Evaluation of Lecithin-Based Nanoparticles for siRNA Delivery. Journal of Drug Delivery،Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-513789
Modern Language Association (MLA)
Pérez, Sebastián Ezequiel…[et al.]. Formulation Strategies, Characterization, and In Vitro Evaluation of Lecithin-Based Nanoparticles for siRNA Delivery. Journal of Drug Delivery No. 2012 (2012), pp.1-9.
https://search.emarefa.net/detail/BIM-513789
American Medical Association (AMA)
Pérez, Sebastián Ezequiel& Gándola, Yamila B.& Carlucci, Adriana Mónica& Gonzalez, Lorena& Turyn, Daniel& Bregni, Carlos. Formulation Strategies, Characterization, and In Vitro Evaluation of Lecithin-Based Nanoparticles for siRNA Delivery. Journal of Drug Delivery. 2012. Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-513789
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-513789