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Partial Purification and Characterization of the Mode of Action of Enterocin S37 : A Bacteriocin Produced by Enterococcus faecalis S37 Isolated from Poultry Feces
Joint Authors
Choiset, Y.
Dalgalarrondo, M.
Belguesmia, Y.
Chobert, J.-M.
Drider, D.
Prévost, H.
Source
Journal of Environmental and Public Health
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-08-02
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
The aim of this research was to purify and characterize the mode of action of enterocin S37, a bacteriocin produced by Enterococcus faecalis S37, a strain recently isolated from the chicken feces.
Enterocin S37 has a molecular weight comprised between 4 and 5 kDa.
It remained active after 1 h at 80oC and at pH values ranging from 4.0 to 9.0.
Furthermore, cell-free supernatant of Enterococcus faecalis S37 and purified enterocin S37 were active against Gram-positive bacteria including Listeria monocytogenes EGDe, L.
innocua F, Enterococcus faecalis JH2-2, and Lactobacillus brevis F145.
The purification of enterocin S37 was performed by ammonium sulfate precipitation followed up by hydrophobic-interaction chromatography procedures.
Treatment of enterocin S37 with proteinase K, α-chymotrypsin, and papain confirmed its proteinaceous nature, while its treatment with lysozyme and lipase resulted in no alteration of activity.
Enterocin S37 is hydrophobic, anti-Listeria and likely acting by depletion of intracellular K+ ions upon action on KATP channels.
This study contributed to gain more insights into the mode of action of enterocins.
American Psychological Association (APA)
Belguesmia, Y.& Choiset, Y.& Prévost, H.& Dalgalarrondo, M.& Chobert, J.-M.& Drider, D.. 2010. Partial Purification and Characterization of the Mode of Action of Enterocin S37 : A Bacteriocin Produced by Enterococcus faecalis S37 Isolated from Poultry Feces. Journal of Environmental and Public Health،Vol. 2010, no. 2010, pp.1-8.
https://search.emarefa.net/detail/BIM-513807
Modern Language Association (MLA)
Belguesmia, Y.…[et al.]. Partial Purification and Characterization of the Mode of Action of Enterocin S37 : A Bacteriocin Produced by Enterococcus faecalis S37 Isolated from Poultry Feces. Journal of Environmental and Public Health No. 2010 (2010), pp.1-8.
https://search.emarefa.net/detail/BIM-513807
American Medical Association (AMA)
Belguesmia, Y.& Choiset, Y.& Prévost, H.& Dalgalarrondo, M.& Chobert, J.-M.& Drider, D.. Partial Purification and Characterization of the Mode of Action of Enterocin S37 : A Bacteriocin Produced by Enterococcus faecalis S37 Isolated from Poultry Feces. Journal of Environmental and Public Health. 2010. Vol. 2010, no. 2010, pp.1-8.
https://search.emarefa.net/detail/BIM-513807
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-513807