Purification and Characterization of a Thermostable Lipase from Geobacillus thermodenitrificans IBRL-nra
Joint Authors
Ahmad Rashid, Fatimah Azzahra
Balan, Anuradha
Ibrahim, Darah
Abdul Rahim, Rashidah
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-11-11
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Thermostable lipase from Geobacillus thermodenitrificans IBRL-nra was purified and characterized.
The production of thermostable lipase from Geobacillus thermodenitrificans IBRL-nra was carried out in a shake-flask system at 65°C in cultivation medium containing; glucose 1.0% (w/v); yeast extract 1.25% (w/v); NaCl 0.45% (w/v) olive oil 0.1% (v/v) with agitation of 200 rpm for 24 hours.
The extracted extracellular crude thermostable lipase was purified to homogeneity by using ultrafiltration, Heparin-affinity chromatography, and Sephadex G-100 gel-filtration chromatography by 34 times with a final yield of 9%.
The molecular weight of the purified enzyme was estimated to be 30 kDa after SDS-PAGE analysis.
The optimal temperature for thermostable lipase was 65°C and it retained its initial activity for 3 hours.
Thermostable lipase activity was highest at pH 7.0 and stable for 16 hours at this pH at 65°C.
Thermostable lipase showed elevated activity when pretreated with BaCl2, CaCl2, and KCl with 112%, 108%, and 106%, respectively.
Lipase hydrolyzed tripalmitin (C16) and olive oil with optimal activity (100%) compared to other substrates.
American Psychological Association (APA)
Balan, Anuradha& Ibrahim, Darah& Abdul Rahim, Rashidah& Ahmad Rashid, Fatimah Azzahra. 2012. Purification and Characterization of a Thermostable Lipase from Geobacillus thermodenitrificans IBRL-nra. Enzyme Research،Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-513911
Modern Language Association (MLA)
Balan, Anuradha…[et al.]. Purification and Characterization of a Thermostable Lipase from Geobacillus thermodenitrificans IBRL-nra. Enzyme Research No. 2012 (2012), pp.1-7.
https://search.emarefa.net/detail/BIM-513911
American Medical Association (AMA)
Balan, Anuradha& Ibrahim, Darah& Abdul Rahim, Rashidah& Ahmad Rashid, Fatimah Azzahra. Purification and Characterization of a Thermostable Lipase from Geobacillus thermodenitrificans IBRL-nra. Enzyme Research. 2012. Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-513911
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-513911