FGF Receptor-Mediated Gene Delivery Using Ligands Coupled to PEI-β-CyD
Joint Authors
Hu, Yiping
Cheng, Wenxiang
Zhang, Peng
Tang, Guping
Li, Jinchao
Liu, Jun
Yue, Ye
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-04-11
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
A novel vector with high gene delivery efficiency and special cell-targeting ability was developed using a good strategy that utilized low-molecular-weight polyethylenimine (PEI; molecular weight: 600 KDa [PEI600]) crosslinked to β-cyclodextrin (β-CyD) via a facile synthetic route.
Fibroblast growth factor receptors (FGFRs) are highly expressed in a variety of human cancer cells and are potential targets for cancer therapy.
In this paper, CY11 peptides, which have been proven to combine especially with FGFRs on cell membranes were coupled to PEI-β-CyD using N-succinimidyl-3-(2-pyridyldithio) propionate as a linker.
The ratios of PEI600, β-CyD, and peptide were calculated based on proton integral values obtained from the 1H-NMR spectra of the resulting products.
Electron microscope observations showed that CY11-PEI-β-CyD can efficiently condense plasmid DNA (pDNA) into nanoparticles of about 200 nm, and MTT assays suggested the decreased toxicity of the polymer.
Experiments on gene delivery efficiency in vitro showed that CY11-PEI-β-CyD/pDNA polyplexes had significantly greater transgene activities than PEI-β-CyD/pDNA in the COS-7 and HepG2 cells, which positively expressed FGFR, whereas no such effect was observed in the PC-3 cells, which negatively expressed FGFR.
Our current research indicated that the synthesized nonviral vector shows improved gene delivery efficiency and targeting specificity in FGFR-positive cells.
American Psychological Association (APA)
Hu, Yiping& Tang, Guping& Liu, Jun& Cheng, Wenxiang& Yue, Ye& Li, Jinchao…[et al.]. 2012. FGF Receptor-Mediated Gene Delivery Using Ligands Coupled to PEI-β-CyD. Journal of Biomedicine and Biotechnology،Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-513976
Modern Language Association (MLA)
Hu, Yiping…[et al.]. FGF Receptor-Mediated Gene Delivery Using Ligands Coupled to PEI-β-CyD. Journal of Biomedicine and Biotechnology No. 2012 (2012), pp.1-7.
https://search.emarefa.net/detail/BIM-513976
American Medical Association (AMA)
Hu, Yiping& Tang, Guping& Liu, Jun& Cheng, Wenxiang& Yue, Ye& Li, Jinchao…[et al.]. FGF Receptor-Mediated Gene Delivery Using Ligands Coupled to PEI-β-CyD. Journal of Biomedicine and Biotechnology. 2012. Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-513976
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-513976