Agrobacterium tumefaciens T-DNA Integration and Gene Targeting in Arabidopsis thaliana Non-Homologous End-Joining Mutants
Joint Authors
Jia, Qi
Bundock, Paul
Hooykaas, Paul J. J.
de Pater, Sylvia
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-02-29
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
In order to study the role of AtKu70 and AtKu80 in Agrobacterium-mediated transformation and gene targeting, plant lines with a T-DNA insertion in AtKu80 or AtKu70 genes were functionally characterized.
Such plant lines lacked both subunits, indicating that heterodimer formation between AtKu70 and AtKu80 is needed for the stability of the proteins.
Homozygous mutants were phenotypically indistinguishable from wild-type plants and were fertile.
However, they were hypersensitive to the genotoxic agent bleomycin, resulting in more DSBs as quantified in comet assays.
They had lower end-joining efficiency, suggesting that NHEJ is a critical pathway for DSB repair in plants.
Both Atku mutants and a previously isolated Atmre11 mutant were impaired in Agrobacterium T-DNA integration via floral dip transformation, indicating that AtKu70, AtKu80, and AtMre11 play an important role in T-DNA integration in Arabidopsis.
The frequency of gene targeting was not significantly increased in the Atku80 and Atku70 mutants, but it was increased at least 10-fold in the Atmre11 mutant compared with the wild type.
American Psychological Association (APA)
Jia, Qi& Bundock, Paul& Hooykaas, Paul J. J.& de Pater, Sylvia. 2012. Agrobacterium tumefaciens T-DNA Integration and Gene Targeting in Arabidopsis thaliana Non-Homologous End-Joining Mutants. Journal of Botany،Vol. 2012, no. 2012, pp.1-13.
https://search.emarefa.net/detail/BIM-513982
Modern Language Association (MLA)
Jia, Qi…[et al.]. Agrobacterium tumefaciens T-DNA Integration and Gene Targeting in Arabidopsis thaliana Non-Homologous End-Joining Mutants. Journal of Botany No. 2012 (2012), pp.1-13.
https://search.emarefa.net/detail/BIM-513982
American Medical Association (AMA)
Jia, Qi& Bundock, Paul& Hooykaas, Paul J. J.& de Pater, Sylvia. Agrobacterium tumefaciens T-DNA Integration and Gene Targeting in Arabidopsis thaliana Non-Homologous End-Joining Mutants. Journal of Botany. 2012. Vol. 2012, no. 2012, pp.1-13.
https://search.emarefa.net/detail/BIM-513982
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-513982