![](/images/graphics-bg.png)
Effects of In Vitro Maturation on Histone Acetylation in Metaphase II Oocytes and Early Cleavage Embryos
Joint Authors
Xu, Chen-Ming
Zhan, Qi-Tao
Li, Li
Le, Fang
Ding, Guo-Lian
Jin, Fan
Jiang, Shi-Wen
Dong, Min-Yue
Huang, He-Feng
Wang, Ning
Source
Obstetrics and Gynecology International
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-06-20
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
In vitro maturation (IVM) of oocyte is an effective procedure for avoiding ovarian hyperstimulation syndrome in patients with polycystic ovaries (PCOS) during in vitro fertilization (IVF).
To investigate the influences of IVM on epigenetic reprogramming and to search for the possible reasons for the lower rates of fertilization and cleavage in IVM oocytes, we examined the expression of two enzymes controlling histone acetylation, histone acetyltransferase GCN5 (GCN5) and histone deacetylase 1 (HDAC1), as well as their common target, acetyl-histone H3 (Ac-H3), in mouse metaphase II (MII) oocytes and preimplantation embryos.
Results showed that IVM downregulated the protein expression of GCN5 in MII oocytes and two-cell embryos and changed the distribution of GCN5 in two-cell embryos.
Expression of HDAC1 mRNA in MII oocytes and two-cell embryos decreased in the IVM group.
However, none of these changes persisted after two-cell embryos.
Levels of Ac-H3 in both oocytes and embryos remained unchanged after IVM.
Our studies indicated that IVM could affect the protein and gene expression related to histone acetylation in oocytes and early cleavage embryos.
By function of selection, parts of the changes could be recovered in late embryo development.
American Psychological Association (APA)
Wang, Ning& Le, Fang& Zhan, Qi-Tao& Li, Li& Dong, Min-Yue& Ding, Guo-Lian…[et al.]. 2010. Effects of In Vitro Maturation on Histone Acetylation in Metaphase II Oocytes and Early Cleavage Embryos. Obstetrics and Gynecology International،Vol. 2010, no. 2010, pp.1-9.
https://search.emarefa.net/detail/BIM-513983
Modern Language Association (MLA)
Wang, Ning…[et al.]. Effects of In Vitro Maturation on Histone Acetylation in Metaphase II Oocytes and Early Cleavage Embryos. Obstetrics and Gynecology International No. 2010 (2010), pp.1-9.
https://search.emarefa.net/detail/BIM-513983
American Medical Association (AMA)
Wang, Ning& Le, Fang& Zhan, Qi-Tao& Li, Li& Dong, Min-Yue& Ding, Guo-Lian…[et al.]. Effects of In Vitro Maturation on Histone Acetylation in Metaphase II Oocytes and Early Cleavage Embryos. Obstetrics and Gynecology International. 2010. Vol. 2010, no. 2010, pp.1-9.
https://search.emarefa.net/detail/BIM-513983
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-513983