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Group VIB Phospholipase A2 Promotes Proliferation of INS-1 Insulinoma Cells and Attenuates Lipid Peroxidation and Apoptosis Induced by Inflammatory Cytokines and Oxidant Agents
Joint Authors
Song, Haowei
Tan, Min
Bao, Shunzhong
Turk, John
Wohltmann, Mary
Ladenson, Jack H.
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-11-11
Country of Publication
Egypt
No. of Pages
16
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
Group VIB Phospholipase A2 (iPLA2γ) is distributed in membranous organelles in which β-oxidation occurs, that is, mitochondria and peroxisomes, and is expressed by insulin-secreting pancreatic islet β-cells and INS-1 insulinoma cells, which can be injured by inflammatory cytokines, for example, IL-1β and IFN-γ, and by oxidants, for example, streptozotocin (STZ) or t-butyl-hydroperoxide (TBHP), via processes pertinent to mechanisms of β-cell loss in types 1 and 2 diabetes mellitus.
We find that incubating INS-1 cells with IL-1β and IFN-γ, with STZ, or with TBHP causes increased expression of iPLA2γ mRNA and protein.
We prepared INS-1 knockdown (KD) cell lines with reduced iPLA2γ expression, and they proliferate more slowly than control INS-1 cells and undergo increased membrane peroxidation in response to cytokines or oxidants.
Accumulation of oxidized phospholipid molecular species in STZ-treated INS-1 cells was demonstrated by LC/MS/MS scanning, and the levels in iPLA2γ-KD cells exceeded those in control cells.
iPLA2γ-KD INS-1 cells also exhibited higher levels of apoptosis than control cells when incubated with STZ or with IL-1β and IFN-γ.
These findings suggest that iPLA2γ promotes β-cell proliferation and that its expression is increased during inflammation or oxidative stress as a mechanism to mitigate membrane injury that may enhance β-cell survival.
American Psychological Association (APA)
Bao, Shunzhong& Song, Haowei& Tan, Min& Wohltmann, Mary& Ladenson, Jack H.& Turk, John. 2012. Group VIB Phospholipase A2 Promotes Proliferation of INS-1 Insulinoma Cells and Attenuates Lipid Peroxidation and Apoptosis Induced by Inflammatory Cytokines and Oxidant Agents. Oxidative Medicine and Cellular Longevity،Vol. 2012, no. 2012, pp.1-16.
https://search.emarefa.net/detail/BIM-513993
Modern Language Association (MLA)
Bao, Shunzhong…[et al.]. Group VIB Phospholipase A2 Promotes Proliferation of INS-1 Insulinoma Cells and Attenuates Lipid Peroxidation and Apoptosis Induced by Inflammatory Cytokines and Oxidant Agents. Oxidative Medicine and Cellular Longevity No. 2012 (2012), pp.1-16.
https://search.emarefa.net/detail/BIM-513993
American Medical Association (AMA)
Bao, Shunzhong& Song, Haowei& Tan, Min& Wohltmann, Mary& Ladenson, Jack H.& Turk, John. Group VIB Phospholipase A2 Promotes Proliferation of INS-1 Insulinoma Cells and Attenuates Lipid Peroxidation and Apoptosis Induced by Inflammatory Cytokines and Oxidant Agents. Oxidative Medicine and Cellular Longevity. 2012. Vol. 2012, no. 2012, pp.1-16.
https://search.emarefa.net/detail/BIM-513993
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-513993