Biochemical studies on antibiotic production from Streptomyces sp : taxonomy, fermentation, isolation and biological properties

Abstract EN

Tunicamycin is a nucleotide antibiotic which was isolated from the fermentation broth of a Streptomyces strain No.

T-4.

According to the morphological, cultural, physiological and biochemical characteristics, and 16S rDNA sequence analysis, strain T-4 was identified as Streptomyces torulosus.

It is active in vitro against some microbial pathogenic viz: Staphylococcus aureus, NCTC 7447; Micrococcus lutea, ATCC 9341 ; Bacillus subtilis, NCTC 10400 ; B.

pumilus, NCTC ; Klebsiella pneumonia, NCIMB 9111 ; Escherichia coli, NCTC 10416 ; Pseudomonas aeruginosa, ATCC 10145 ; Saccharomyces cerevisiae ATCC 9763 ; Candida albicans, IMRU 3669 ; Aspergillus flavus, IMI 111023 ; Aspergillus niger IMI 31276 ; Aspergillus fumigatus ATCC 16424 ; Fusarium oxysporum ; Rhizoctonia solani ; Alternaria alternata ; Botrytis fabae and Penicillium chrysogenium.

The production media were optimized for maximum yield of secondary metabolites.

The metabolites were extracted using nbutanol (1 : 1, v / v) at pH 7.0.

The chemical structural analysis with UV, IR, and MS spectral analyses confirmed that the compound produced by Streptomyces torulosus, T-4 is tunicamycin antibiotic.