Evaluation of different in-vitro culture media for cultivation of leishmania parasites
Joint Authors
Alfaki, Taysir al-Amin Muhammad
Abd al-Karim, Amjad Muhammad
al-Amin, al-Walid Muhammad
Source
Journal of Science and Technology : in Natural and Medical Sciences
Issue
Vol. 2014, Issue (sup) (31 Dec. 2014), pp.35-43, 9 p.
Publisher
Sudan University of Science and Technology Deanship of Scientific Research
Publication Date
2014-12-31
Country of Publication
Sudan
No. of Pages
9
Main Subjects
Topics
Abstract AR
اجریت هذه الد ا رسة بمعهد الام ا رض المتوطنة، جامعة الخرطوم خلال الفترة من ینایر 2007 إلى دیسمبر Novy - MacNeal - Nicolle 2007 م.
قارنت الدراسة بین دم الحصان و دم الارنب كمكون للوسط الغذائى لتزریع طفیل اللشمانیا.
تم تزریع Lowestein Jensen (LJ) بالإضافة لإستخدام الوسط الغذائى (NNN) 5% ) و اخرى تحتوى على , 10% and الطفیل فى انابیب تحتوى على ت ا ركیز مختلفة من دم الحصان (% 20 كل الانابیب حضنت لمدة 48 ساعة فى درجة ح ا ررة .LJ دم الارنب بتركیز (% 10 ) كما تم تزریعھ فى الوسط 23 درجة مئویة.
وتم حساب اعداد الطفیل كل 48 ساعة.
الذى یحتوى على دم الحصان (خاصة NNN اظهرت هذه الدراسة أن معدل نمو اللشمانیا یكون فى الوسط .LJ التركیز % 10 ) و دم الارنب.
دم الارنب اظهر نمو افضل من دم الحصان.
و لم یحدث النمو فى الوسط
Abstract EN
This study was held in the Institute of Endemic Diseases, University of Khartoum during the period from January 2007 to December 2007.
The study compared between horse blood and rabbit blood as main constituent of Novy-MacNeal-Nicolle (NNN) culture media in addition to the use of Lowestein Jensen (LJ) media for cultivation of Leishmania parasite.
The parasite was inoculated in tubes contained different concentrations of horse blood (5%, 10% and 20%) and other tubes contained rabbit blood with a concentration of 10%, also the parasite was inoculated in LJ media.
All the tubes were incubated for 48 hours at 23 ºC.
Parasites were then counted every 48 hours.
This study indicated that Leishmania parasite showed the growth rate in the NNN media which contains horse blood (especially 10%) and rabbit blood.
The rabbit blood showed better growth than horse blood.
There was no growth in the LJ medium.
American Psychological Association (APA)
Alfaki, Taysir al-Amin Muhammad& Abd al-Karim, Amjad Muhammad& al-Amin, al-Walid Muhammad. 2014. Evaluation of different in-vitro culture media for cultivation of leishmania parasites. Journal of Science and Technology : in Natural and Medical Sciences،Vol. 2014, no. (sup), pp.35-43.
https://search.emarefa.net/detail/BIM-520852
Modern Language Association (MLA)
Alfaki, Taysir al-Amin Muhammad…[et al.]. Evaluation of different in-vitro culture media for cultivation of leishmania parasites. Journal of Science and Technology : in Natural and Medical Sciences (2014), pp.35-43.
https://search.emarefa.net/detail/BIM-520852
American Medical Association (AMA)
Alfaki, Taysir al-Amin Muhammad& Abd al-Karim, Amjad Muhammad& al-Amin, al-Walid Muhammad. Evaluation of different in-vitro culture media for cultivation of leishmania parasites. Journal of Science and Technology : in Natural and Medical Sciences. 2014. Vol. 2014, no. (sup), pp.35-43.
https://search.emarefa.net/detail/BIM-520852
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 41-43
Record ID
BIM-520852
