Prevalence and characterization of Salmonella species isolated from broilers

Abstract EN

This study was conducted to determine the prevalence of Salmonellae in broilers farms in Dakahlia Governorate, Egypt.

A total of 1000 samples that collected from 200 broiler chickens (40 apparently healthy, 80 diseased chickens and 80 freshly dead broiler chickens).These samples included liver, caecum, heart blood, spleen & kidney.

The colonial morphology, microscpical and biochemical identifications of the isolates revealed the presence of 37 Salmonella isolates out of 200 chickens (18.5 %) representing : 3 from apparently healthy chicken (7.5 %), 21 from diseased chickens (26.25 %) and 13 from freshly dead broiler chickens (16.25 %).

The rate of recovery of Salmonellae from the different internal organs showed that high recovery rate was from liver, caecum, spleen, heart then kidney as the follow (9.5 %), (5.5 %), (4.5 %), (3 %) and (2 %), respectively.

The serotyping of the isolated salmonellae from chickens were eight S.

enteritidis, one S.

maccles Field, two S.

wingrove, one S.

eingedi, three S.

rissen, two S.

derby, two S.

vejle, one S.

magherafelt, two S.

berta, two S.

enterica sub.spp salamae, one S.

gueuletapee, one S.

blegdam, five S.

kentucky, two S.

newport, two S.

agona and two S.

virchow.

Gentamycin, ciprofloxacin, colistin sulphate and enrofloxacin were found to be the most effective antimicrobials drugs while erythromycin and flumequine were the most resistant antibiotic against the isolates.

PCR assay was carried out for six serovars (S.

enteritidis, S.

maccles Field, S.

rissen, S.

derby, S.

magherafelt and S.

enterica sub.spp salamae) to detect the presence of invA, sopB and stn genes.

All serovars had the three genes.