Bacteriological and molecular studies on salmonella in chickens

Abstract EN

A total 396 samples from 132 broiler showed diarrhea and common clinical signs (samples from liver, heart blood and intestinal content) were collected from Port Said governorate.

All samples were subjected for isolation and identification of salmonella.

Salmonella species were recovered in 121 (30.5 %) from all examined samples according to the traditional methods.

25 samples from broiler chicken which showed typical post mortem picture for salmonella infection and confirmed by biochemically traditional methods were subjected to API 20E system for qualification that rapid test.

Serotyping of Salmonella isolates could be identified into Salmonella enteritidis (1, 9, 12 ; g, m ;), Salmonella kentuckey (8, 20 ; I ; z6), Salmonella bloemfontein (6, 7 ; e, n, x, z42) and Salmonella archavalenta (4, (15), 12, a ; 1, 7).

The result of sensitivity of the four Salmonella strains to 13 antimicrobial agents revealed that Salmonella isolates were sensitive to choloramphenicol, gentamycin, ofloxacin variable to ampicillin, enrofloxacin, naldixic acid, sulfa / trimethoprim and resistant to erythromycin and lincomycin.

Theses identified Serotyping of Salmonella were further confirmed using polymerase chain reaction technique and SYBR Green real-time PCR for detection of Salmonella invA gene that present only in Salmonella species as a rapid and more sensitive technique.

SYBR Green real-time PCR was conducted to the four identified Salmonella serovars to detect the difference in melting temperature.

It showed that S.

enteitidis Time Product1 (-R'(T) at 82, S.

bloemfontein at 77.63, Salmonella kentucky at 83.51 and Salmonella arechavaleta at 81.92.