Penicillium ochrochloron MTCC 517 chitinase : an effective tool in commercial enzyme cocktail for production and regeneration of protoplasts from various fungi
Joint Authors
Patil, Nilambari S.
Jadhav, Jyoti P.
Source
Saudi Journal of Biological Sciences
Issue
Vol. 22, Issue 2 (30 Jun. 2015), pp.232-236, 5 p.
Publisher
Publication Date
2015-06-30
Country of Publication
Saudi Arabia
No. of Pages
5
Main Subjects
Topics
Abstract EN
Penicillium ochrochloron MTCC 517 is a potent producer of chitinolytic enzymes.
Novozyme 234, traditional enzyme cocktail for protoplast generation is not available in the market.
So, new enzyme cocktail is prepared for protoplast formation from various filamentous fungi which consists of 5 mg ml-1 lysing enzymes from Trichoderma harzianum, 0.06 mg ml-1 β-glucuronidase from Helix pomatia and 1 mg ml-1 P.
ochrochloron chitinase.
The greatest number of protoplasts could be produced from most of the fungi in 0.8 M sorbitol and by incubation for about 2 h at 37°C, but the number was decreased by incubation for more than 3 h.
About twice as many protoplasts were produced from different species of fungi by involvement of P.
ochrochloron chitinase than with combined commercial enzymes.
American Psychological Association (APA)
Patil, Nilambari S.& Jadhav, Jyoti P.. 2015. Penicillium ochrochloron MTCC 517 chitinase : an effective tool in commercial enzyme cocktail for production and regeneration of protoplasts from various fungi. Saudi Journal of Biological Sciences،Vol. 22, no. 2, pp.232-236.
https://search.emarefa.net/detail/BIM-527491
Modern Language Association (MLA)
Patil, Nilambari S.& Jadhav, Jyoti P.. Penicillium ochrochloron MTCC 517 chitinase : an effective tool in commercial enzyme cocktail for production and regeneration of protoplasts from various fungi. Saudi Journal of Biological Sciences Vol. 22, no. 2 (2015), pp.232-236.
https://search.emarefa.net/detail/BIM-527491
American Medical Association (AMA)
Patil, Nilambari S.& Jadhav, Jyoti P.. Penicillium ochrochloron MTCC 517 chitinase : an effective tool in commercial enzyme cocktail for production and regeneration of protoplasts from various fungi. Saudi Journal of Biological Sciences. 2015. Vol. 22, no. 2, pp.232-236.
https://search.emarefa.net/detail/BIM-527491
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 235-236
Record ID
BIM-527491