Numerical analysis of whole cell protein of local salmonella enterica serovar typhi from human blood

Abstract EN

Twenty nine isolates of Salmonella enterica serovar Typhi (S.

typhi) were obtained from the blood of patients suspected to be infected with typhoid fever.

Isolates were biotyped, serotyped, antibiotyped, and characterized by SDS-PAGE of whole cell protein profile (WCPP).

Numerical analysis by use of Jaccard's coefficient (Sj) and UPGMA clustering of the WCPP of the isolates were performed.

All isolates were serologically confirmed and found to belong to polyvalent A-60 and monovalent 9, and were belonged to two different biotypes, and to 12 antibiotypes.

The bacterial WCPP indicated that the individual isolate had 20 to 26 protein bands ranging in their molecular weight from 14 KDa (kilodaltons) to 400.

The numerical analysis of WCPP revealed three major distinct clusters (A, B, C), produced at (Sj 60%), only two isolates remains clusters, and were linked to them at lower levels.