Application of biotechnology methods for genetic improvement of mahogany tree (Khaya senegalensis L.)‎

Abstract EN

Biotechnology has the potential to accelerate the genetic improvement of forest yield and cloning of selected genotypes.

A protocol for in vitro micropropagation for improving mahogany (Khaya senegalensis) using MS salts (Murashige & Skoog, 1962) containing 3 mg / l MS vitamins, 0.5 mg / l Benziladenine (BA), 0.5-5.0 mg / L Kinetin in combination with Auxins : Navthaleneacetic acid (NAA) and 3 mg / L 2,4-dichlorophenoxyacetic acid (2,4-D) for callus induction, 6 % sucrose and 7 g / l agar (Sigma) with pH adjusted to (5.7).

120-2 20 shoots were obtained from each original bud explant after 6-8 weeks.

Culturing explants on hormone-free media involves the development of the nodular tissue formed during bud induction into shoots with primary needles.

On the other hand, the addition of activated charcoal and lowering the sucrose level to 1-2 % enhance shoot elongation.

Callus induced effectively with 2,4-D.

Shoot production increased with increasing Benzyladenine (BA) concentration (from 0 to 4.0 mg / l).

Kinetin in combination with BA produced more vigorous shoots than BA used singly.

The number of shoots increased as the BA concentration increased from 0 to 4 mg / l at both the 1st and 4th recultures.

There were highly significant differences between the different concentrations.

Also results in a proportional increase in shoot vitrification.

The effect was more pronounced at high BA concentration.

Increasing BA concentrations promoted the percentage of vitrified shoots particularly at the 4th reculture.