Derivatization chromophore introduction of tranexamic acid and its hplc determination in pharmaceutical formulations
Joint Authors
Ashfaq, M.
Aslam, A.
Mustafa, G.
Danish, M.
Nazar, M. Faizan
Asghar, M. Nadim
Source
Arab Journal of Basic and Applied Sciences
Issue
Vol. 17, Issue 0 (30 Apr. 2015), pp.51-56, 6 p.
Publisher
University of Bahrain College of Science
Publication Date
2015-04-30
Country of Publication
Bahrain
No. of Pages
6
Main Subjects
Abstract EN
A viable cost-effective and isocratic approach employing C-18 column (250 mm · 4.6 mm, 5 lm) based HPLC has been utilized to separate and estimate the drug, tranexamic acid in pharmaceutical formulations.
Tranexamic acid contains no p-electrons to act as fluorophore or chromophore hence pre-column derivatization was performed with benzene sulfonyl chloride in aqueous medium at room temperature.
The derivatized drug was then estimated using C-18 column by exploiting a 25:75 (v/v) solvent mixture of acetonitrile and 0.1 M ammonium acetate (pH 5.0) as the mobile phase.
The flow rate of mobile phase was 1 mL/min and detection was performed at a wavelength of 232 nm using UV detector.
Retention time of tranexamic acid was 4.42 min.
The method followed linear regression equation in the concentration range of 1– 100 lg/mL with co-efficient of determination equal to 0.9994.
The limit of detection and limit of quantitation were 0.3 and 1 lg/mL, respectively.
The relative standard deviation and recovery ranges for tranexamic acid were found to be 0.11–2.47% and 97.60–103.25%, respectively.
The suggested method is very sensitive and may have the potential to be used for tranexamic acid detection in medicinal formulations.
American Psychological Association (APA)
Ashfaq, M.& Aslam, A.& Mustafa, G.& Danish, M.& Nazar, M. Faizan& Asghar, M. Nadim. 2015. Derivatization chromophore introduction of tranexamic acid and its hplc determination in pharmaceutical formulations. Arab Journal of Basic and Applied Sciences،Vol. 17, no. 0, pp.51-56.
https://search.emarefa.net/detail/BIM-535346
Modern Language Association (MLA)
Ashfaq, M.…[et al.]. Derivatization chromophore introduction of tranexamic acid and its hplc determination in pharmaceutical formulations. Arab Journal of Basic and Applied Sciences Vol. 17 (Apr. 2015), pp.51-56.
https://search.emarefa.net/detail/BIM-535346
American Medical Association (AMA)
Ashfaq, M.& Aslam, A.& Mustafa, G.& Danish, M.& Nazar, M. Faizan& Asghar, M. Nadim. Derivatization chromophore introduction of tranexamic acid and its hplc determination in pharmaceutical formulations. Arab Journal of Basic and Applied Sciences. 2015. Vol. 17, no. 0, pp.51-56.
https://search.emarefa.net/detail/BIM-535346
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 55-56
Record ID
BIM-535346