The influence of iron oxide nanoparticles on the red blood cells photohemolysis sensitized with photofrin: temperature effect

Abstract EN

The present study aims to measure the temperature-dependence of in vitro continuous photohemolysis (CPH) photosensitized by Photofrin® in the presence or absence of Iron Oxide Nanoparticles (IONPs) and to evaluate the results using Gompertz function.

Red Blood Cells (RBCs) were isolated from human healthy volunteers blood; they were then incubated with Photofrin® only or with IONPs for 45 minutes at 37 °C and then irradiated to a range of temperatures (4- 41°C).

The results show that Photosensitization of RBCs by Photofrin® with IONPs presence reduces the inhabitation effect of Photofrin® at the same irradiation temperature since the decreasing in activation energy and increment in tR50R were obvious evidence for such result as well as the applicability of Gompertz function to the fractional photohemolysis ratio (a) and the rate of fractional photohemolysis (b), is found to be the most appropriate model to fit the experimental data with minimum parameters and minimum errors, Parameter (a) and the curves steepness were found to be temperatureindependent.

On the other hand, values of parameter (b) increased as irradiation temperature increased with or without IONPs presence.

The apparent activation energy was found to be 18.85 ± 0.72 kcal/mol in the absence of IONPs and 17.29 ± 0.71 kcal/mol in the presence of IONPs.

Our results indicated that Photofrin® incorporated with IONPs could be considered as a modality to improve cytotoxicity in photodynamic therapy and/or reduces the inhabitation effect.