The effect of aqueous extract and essential oils of the leaves of eucalyptus incrassate on β- lactam resistant staphylococcus aureus

Abstract AR

According to the extraction procedure , Eucalyptus incrassata leave sample yielded 5% and 2% w/w(Based on dry leaves ) of the aqueous extract and essential oils respectively.

Disk diffusion method was used to determine the antimicrobial activity of aqueous extract and essential oils of E .

incrassata leaves against eight isolates of multidrug- resistant of Staphylococcus aureus ( S.

aureus) .

It was found that aqueous extract and essential oils have variable antimicrobial activity(the inhibition zone diameter ranged from 7 to 14 mm respectively ) , while essential oils showed more effect than aqueous extract .

It was noticed that values of Minimal Inhibitory Concentration ( MIC ) for aqueous extract is different from MIC of essential oils and these values were ranged from12.5 to 75mg/ml .

It was also found that isolates collected from wound infections showed high resistance against aqueous extract and essential oils with MIC ranged from 50-75mg/ml, these variation in the MIC are probably due to some virulence factors which have a unique genetic determinant .

The results indicated that the potential usefulness of the aqueous extract and essential oils of Eucalyptus incrassata as an antimicrobial agent can be used in the treatment of multidrug-resistant S.

aureus infectious .