Role of extracted genomic DNA on biofilm formation by Pseudomonas aeruginosa and Klebsiella pneumoniae in vitro
Other Title(s)
دور DNA المستخلص في تكوين الغشاء الحيوي لبكتريا Pseudomonas aeruginosa, Klebsiell pneumonia خارج الجسم الحي
Joint Authors
Ulaywi, Sana Rahman
Abd, Huwayda Karim
Source
Ibn al-Haitham Journal for Pure and Applied Science
Issue
Vol. 27, Issue 3 (31 Dec. 2014), pp.34-44, 11 p.
Publisher
University of Baghdad College of Education for Pure Science / Ibn al-Haitham
Publication Date
2014-12-31
Country of Publication
Iraq
No. of Pages
11
Main Subjects
Topics
Abstract AR
تكون البكتريا معقدا و مجتمعات ملتصقلة بالسطوح بصورة متقنة جدا يسمى الغشاء الحيوي.
يرتبط الغشاء الحيوي ببعض أمراض الإنسان و كذلك يستوطن شتى الأدوات الطبية، ركزت الدراسة الحالية على دور DNA المستخلص على تشكيل الغشاء الحيوي بواسطة P.
aeruginosa و K.
pneumonia.
كانت نسبة عزل هذه البكتريا من مياه الشرب في هذه الدراسة هي 10 % (20 عزلة P.
aeruginosa) و 7 % (14 عزلة K.
pneumonia).
أظهرت النتائج أن جميع عزلات P.aeruginosa و K.
pneumoniae كانت منتجة للطبقة اللزجة لكن بدرجات مختلفة بتكوين المستعمرات السود على وسط اكار أحمر الكونغو : مستعمرات سود غامقة و سود و حمر.
أشارت النتائج إلى أن إضافة DNA إلى الوسط يحفز تشكيل الغشاء الحيوي (سواء كان DNA ذاتي أو خارجي).
Abstract EN
Bacteria form complex and highly elaborate surface adherent communities known as biofilms.
Biofilm have been shown to be associated with several human diseases, and to colonize a wide variety of medical devices.
The current study focuses on contribution of extracted genomic DNA in biofilm formation by P.
aeruginosa and K.
pneumonia isolates.
The percentages of Pseudomonas aeruginosa recovery from drinking water in this study were 10 % (20 positive P.
aeruginosa samples) and K.
pneumonia., 7 % (14 positive K.
pneumonia samples).
The results showed that all P.
aeruginosa and K.
pneumoniae isolates (100 %) were slime producer but in different degrees by forming of black colonies on congored agar : very black colonies, black colonies and red colonies.
Results indicated that addition of extracted genomic DNA to microtitre-plate cultures stimulate biofilm formation (either endogenous or exogenous DNA).
American Psychological Association (APA)
Ulaywi, Sana Rahman& Abd, Huwayda Karim. 2014. Role of extracted genomic DNA on biofilm formation by Pseudomonas aeruginosa and Klebsiella pneumoniae in vitro. Ibn al-Haitham Journal for Pure and Applied Science،Vol. 27, no. 3, pp.34-44.
https://search.emarefa.net/detail/BIM-545909
Modern Language Association (MLA)
Ulaywi, Sana Rahman& Abd, Huwayda Karim. Role of extracted genomic DNA on biofilm formation by Pseudomonas aeruginosa and Klebsiella pneumoniae in vitro. Ibn al-Haitham Journal for Pure and Applied Science Vol. 27, no. 3 (2014), pp.34-44.
https://search.emarefa.net/detail/BIM-545909
American Medical Association (AMA)
Ulaywi, Sana Rahman& Abd, Huwayda Karim. Role of extracted genomic DNA on biofilm formation by Pseudomonas aeruginosa and Klebsiella pneumoniae in vitro. Ibn al-Haitham Journal for Pure and Applied Science. 2014. Vol. 27, no. 3, pp.34-44.
https://search.emarefa.net/detail/BIM-545909
Data Type
Journal Articles
Language
English
Notes
Includes appendices : p. 41-43
Record ID
BIM-545909
