Effect of sample storage temperature and time delay on blood gases, bicarbonate and pH in human arterial blood samples
Joint Authors
Husayni, Ilham Muhammad
Firoozbakhsh, Shahram
Peiman, Suhayl
Safavi, Inayat
Seifirad, Soroush
Sayfi, Zubaydah
Source
Iranian Red Crescent Medical Journal
Issue
Vol. 17, Issue 3 (31 Mar. 2015), pp.1-4, 4 p.
Publisher
Publication Date
2015-03-31
Country of Publication
United Arab Emirates
No. of Pages
4
Main Subjects
Topics
Abstract EN
Results of arterial blood gas analysis can be biased by pre-analytical factors, such as time interval before analysis, temperature during storage and syringe type.
Objectives : To investigate the effects of samples storage temperature and time delay on blood gases, bicarbonate and PH results in human arterial blood samples.
Patients and Methods : 2.5 mL arterial blood samples were drawn from 45 patients via an indwelling Intraarterial catheter.
Each sample was divided into five equal samples and stored in multipurpose tuberculin plastic syringes.
Blood gas analysis was performed on one of five samples as soon as possible.
Four other samples were divided into two groups stored at 22°C and 0°C.
Blood gas analyses were repeated at 30 and 60 minutes after sampling.
Results: PaO2 of the samples stored at 0°C was increased significantly after 60 minutes (P = 0.007).
The PaCO2 of the samples kept for 30 and 60 minutes at 22°C was significantly higher than primary result (P = 0.04, P < 0.001).
In samples stored at 22°C, pH decreased significantly after 30 and 60 minutes (P = 0.017, P = 0.001).
There were no significant differences in other results of samples stored at 0°C or 22°C after 30 or 60 minutes.
Conclusions : In samples stored in plastic syringes, overestimation of PaO2 levels should be noted if samples cooled before analysis.
In samples stored in plastic syringes, it is not necessary to store samples in iced water when analysis delayed up to one hour.
American Psychological Association (APA)
Husayni, Ilham Muhammad& Safavi, Inayat& Sayfi, Zubaydah& Seifirad, Soroush& Firoozbakhsh, Shahram& Peiman, Suhayl. 2015. Effect of sample storage temperature and time delay on blood gases, bicarbonate and pH in human arterial blood samples. Iranian Red Crescent Medical Journal،Vol. 17, no. 3, pp.1-4.
https://search.emarefa.net/detail/BIM-554075
Modern Language Association (MLA)
Husayni, Ilham Muhammad…[et al.]. Effect of sample storage temperature and time delay on blood gases, bicarbonate and pH in human arterial blood samples. Iranian Red Crescent Medical Journal Vol. 17, no. 3 (Mar. 2015), pp.1-4.
https://search.emarefa.net/detail/BIM-554075
American Medical Association (AMA)
Husayni, Ilham Muhammad& Safavi, Inayat& Sayfi, Zubaydah& Seifirad, Soroush& Firoozbakhsh, Shahram& Peiman, Suhayl. Effect of sample storage temperature and time delay on blood gases, bicarbonate and pH in human arterial blood samples. Iranian Red Crescent Medical Journal. 2015. Vol. 17, no. 3, pp.1-4.
https://search.emarefa.net/detail/BIM-554075
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 4
Record ID
BIM-554075