Effect of natural media on the propagation of orchids by tissue culture

Abstract EN

Orchid flowers are the most fascinating and beautiful of all flowers.

Two orchid species belonging to different genera, Laelia anceps and Cymbidium devonianum were used in this study.

Results of this study could be abstracted in the following : In order to reduce the high expenses of the tissue culture technique for economic purposes, MS Medium could be substituted by some natural, cheap and easily available products such as broad bean, wheat corn or rice flour.

Chemical and hormonal analyses of these substances unveiled their richness of almost all elements represented in the standard MS medium, in addition to their content of a naturally balanced phytohormones.

Corn starch could be the source of sugars needed for the explants to grow, and it can be used to some extent instead of the expensive agar.