Antimicrobial activity and phytochemical screening of methanolic extracts of hypericum triquetrifolium
Other Title(s)
فحص النشاطية المضادة للميكروبات و المواد الكيميائية النباتية للمستخلصات الميثانولية لنبات العرن
Dissertant
Thesis advisor
Comitee Members
al-Zarani, Wail Ali
al-Harub, Amir Munawwir
Atruz, Umar Muhammad Falih
University
Mutah University
Faculty
Faculty of Science
Department
Department of Biological Sciences
University Country
Jordan
Degree
Master
Degree Date
2014
English Abstract
Antimicrobial and antioxidant activities of methanolic extract of the aerial parts of Hypericum triquetrifolium was investigated.
Serial dilution and disc diffusion methods were performed to measure the antibacterial activity of this extract against different bacterial strains (S.
aureus, E.
coli, K.
pneumoniae, B.
subtilis and M.
luteus).
The total phenolic content was assessed by Folin-Ciocalteu method.
The amount of the extract required to scavenge 50% of DPPH radical (IC50) was also measured.
The antioxidant capacity was assessed by both DPPH and FRAP methods.
The results of antibacterial assay showed that M.
luteus, B.
subtilis and S.
aureus exhibited the highest sensitivity to the plant extract followed by K.
pneumoniae and E.
coli.
High amount of phenolic compounds (621 mg GA / g plant extract) was found in the plant extract .
According to the DPPH assay, Hypericum triquetrifolium showed high free radical scavenging potential (62.5 mg Trolox / g plant extract).
The trend for ferric ions reducing activitiy was relatively high (6.7 mg Trolox / g plant extract) .
In conclusion, this plant was not only interesting source for antimicrobial agents but also have a considerable amount of antioxidants.
In addition, these findings revealed that the antioxidant capacity of these plant extracts could be attributed to their total phenolic content
Main Subjects
Topics
No. of Pages
37
Table of Contents
Table of contents.
Abstract.
Abstract in Arabic.
Chapter One : Theoretical background.
Chapter Two : Literature review.
Chapter Three : Materials and methods.
Chapter Four : Results, discussion and recommendation results.
References.
American Psychological Association (APA)
al-Utaybi, Mashal. (2014). Antimicrobial activity and phytochemical screening of methanolic extracts of hypericum triquetrifolium. (Master's theses Theses and Dissertations Master). Mutah University, Jordan
https://search.emarefa.net/detail/BIM-561555
Modern Language Association (MLA)
al-Utaybi, Mashal. Antimicrobial activity and phytochemical screening of methanolic extracts of hypericum triquetrifolium. (Master's theses Theses and Dissertations Master). Mutah University. (2014).
https://search.emarefa.net/detail/BIM-561555
American Medical Association (AMA)
al-Utaybi, Mashal. (2014). Antimicrobial activity and phytochemical screening of methanolic extracts of hypericum triquetrifolium. (Master's theses Theses and Dissertations Master). Mutah University, Jordan
https://search.emarefa.net/detail/BIM-561555
Language
English
Data Type
Arab Theses
Record ID
BIM-561555
