Metabolic changes in blood and ovarian follicular fluid in baladi goats as affected by storage time duration

Abstract EN

The purposes of this study were to determine ; 1) complete blood picture and serum metabolites after different times of storage (0, 4 and 8 h) of either whole blood or blood serum ; 2) metabolites concentration in follicular fluids harvested from different-sized follicles; 3) metabolites concentration in follicular fluids after different times of ovarian storage without (0, 4 and 8 h) or with (0 and 8h) holding medium in goats.

Twenty four Baladi goats were slaughtered in this study.

Blood samples were collected before immediate slaughter of goats.

Fifteen pairs of ovaries with double dominance follicles were selected after slaughter and classified to three categories for collection of follicular fluids upon ovarian storage without (0, 4 and 8 h) or with (0 and 8h) holding medium.

After immediate slaughter (0h), follicular fluids were aspirated from two size classes of non-atretic follicles ; subdominance (≤3 mm) and dominance (>4mm).

Moreover, follicular fluids were aspirated from dominance (> 4mm) follicles upon ovarian storage without (0, 4 and 8 h) or with (0 and 8h) holding medium.

Blood/serum and follicular fluid samples remained independent among goats and follicle size classes within goats.

Blood serum and follicular fluid samples were assayed using photometric assays for complete blood picture and metabolites determination of glucose, total protein, albumin, triglycerides and total cholesterol.

Results showed that blood cell picture, serum and follicular fluid metabolites (glucose, cholesterol and triglycerides) were changed over storage time of blood and ovaries.

The extent of differences was time dependent.

Thus, optimized storage time for ovaries containing large follicles is beneficial for embryo development in vitro which positively can affect the outcomes from embryo transferred to animals.