The effect of estrogen usage on eccentric exercise-induced damage in rat testes
Joint Authors
Can, Serpil
Selli, Jale
Buyuk, Basak
Aydin, Sergulen
Kocaaslan, Ramadan
Guvendi, Gulname Findik
Source
Iranian Red Crescent Medical Journal
Issue
Vol. 17, Issue 4 (30 Apr. 2015), pp.1-9, 9 p.
Publisher
Publication Date
2015-04-30
Country of Publication
United Arab Emirates
No. of Pages
9
Main Subjects
Topics
Abstract EN
Background: Recent years, lots of scientific studies are focused on the possible mechanism of inflammatory response and oxidative stress which are the mechanism related with tissue damage and exercise fatigue.
It is well-known that free oxygen radicals may be induced under invitro conditions as well as oxidative stress by exhaustive physical exercise.
Objectives: The aim of this study was to investigate the effects of anabolic steroids in conjunction with exercise in the process of spermatogenesis in the testes, using histological and stereological methods.
Materials and Methods: Thirty-six male Sprague Dawley rats were divided to six groups, including the control group, the eccentric exercise administered group, the estrogen applied group, the estrogen applied and dissected one hour after eccentric exercise group, the no estrogen applied and dissected 48 hours after eccentric exercise group and the estrogen applied and dissected 48 hours after eccentric exercise group.
Eccentric exercise was performed on a motorized rodent treadmill and the estrogen applied groups received daily physiological doses by subcutaneous injections.
Testicular tissues were examined using specific histopathological, immunohistochemical and stereological methods.
Sections of the testes tissue were stained using the TUNEL method to identify apoptotic cells.
Apoptosis was calculated as the percentage of positive cells, using stereological analysis.
A statistical analysis of the data was carried out with one-way analysis of variance (ANOVA) for the data obtained from stereological analysis.
Results: Conventional light microscopic results revealed that testes tissues of the eccentric exercise administered group and the estrogen supplemented group exhibited slight impairment.
In groups that were both eccentrically exercised and estrogen supplemented, more deterioration was detected in testes tissues.
Likewise, immunohistochemistry findings were also more prominent in the eccentrically exercised and estrogen supplemented groups.
Conclusions: The findings suggest that estrogen supplementation increases damage in testicular tissue due to eccentric exercise.
American Psychological Association (APA)
Can, Serpil& Selli, Jale& Buyuk, Basak& Aydin, Sergulen& Kocaaslan, Ramadan& Guvendi, Gulname Findik. 2015. The effect of estrogen usage on eccentric exercise-induced damage in rat testes. Iranian Red Crescent Medical Journal،Vol. 17, no. 4, pp.1-9.
https://search.emarefa.net/detail/BIM-564815
Modern Language Association (MLA)
Can, Serpil…[et al.]. The effect of estrogen usage on eccentric exercise-induced damage in rat testes. Iranian Red Crescent Medical Journal Vol. 17, no. 4 (Apr. 2015), pp.1-9.
https://search.emarefa.net/detail/BIM-564815
American Medical Association (AMA)
Can, Serpil& Selli, Jale& Buyuk, Basak& Aydin, Sergulen& Kocaaslan, Ramadan& Guvendi, Gulname Findik. The effect of estrogen usage on eccentric exercise-induced damage in rat testes. Iranian Red Crescent Medical Journal. 2015. Vol. 17, no. 4, pp.1-9.
https://search.emarefa.net/detail/BIM-564815
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 8-9
Record ID
BIM-564815