Detection of optimum pH of Momordica balsamina seeds lectin
Joint Authors
Ibrahim, M. A.
Muhammad, M. E.
Said B. O.
Konozy E. H.
al-Hajj A. K.
Ahmad, S. M.
Source
Sudan Journal of Medical Sciences
Issue
Vol. 9, Issue 4 (31 Dec. 2014), pp.217-220, 4 p.
Publisher
Omdurman Islamic University Faculty of Medicine
Publication Date
2014-12-31
Country of Publication
Sudan
No. of Pages
4
Main Subjects
Topics
Abstract EN
Background : Lectins are carbohydrate binding proteins of non-immune origin that reversibly and non-enzymatically bind carbohydrates with high specificity for the chemical structure of the glycan array without changing their structure.
Objectives : The present study aimed to detect the optimum pH of Momordica balsamina seeds lectin (MbSL).
Materials and Methods : A season fresh of Momordica balsaminafruit seeds were brought from urban areas of Sudan (Gadarif and north Kurdofan states), then the lectin was isolated from saline extract by affinity chromatography on alpha agarose lactose matrix then the purified lectin activity was evaluated in different buffers to detect the optimum pH.
Results : The activity of the lectin remained stable in the pH range 2-12.
Conclusion : A lactose-binding lectin from seeds of Momordica balsamina medicinal plant shares a high degree of similarity with other Cucurbitaceae family lectins in term of their physicochemical features including sugar specificity, effect of pH on lectin stability.
American Psychological Association (APA)
Ibrahim, M. A.& Said B. O.& Ahmad, S. M.& Konozy E. H.& Muhammad, M. E.& al-Hajj A. K.. 2014. Detection of optimum pH of Momordica balsamina seeds lectin. Sudan Journal of Medical Sciences،Vol. 9, no. 4, pp.217-220.
https://search.emarefa.net/detail/BIM-565605
Modern Language Association (MLA)
Said B. O.…[et al.]. Detection of optimum pH of Momordica balsamina seeds lectin. Sudan Journal of Medical Sciences Vol. 9, no. 4 (Dec. 2014), pp.217-220.
https://search.emarefa.net/detail/BIM-565605
American Medical Association (AMA)
Ibrahim, M. A.& Said B. O.& Ahmad, S. M.& Konozy E. H.& Muhammad, M. E.& al-Hajj A. K.. Detection of optimum pH of Momordica balsamina seeds lectin. Sudan Journal of Medical Sciences. 2014. Vol. 9, no. 4, pp.217-220.
https://search.emarefa.net/detail/BIM-565605
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 219-220
Record ID
BIM-565605