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Assessment of genetic diversity among faba bean genotypes using agro-morphological and molecular markers
Joint Authors
Ammar, Mujahid H.
al-Ghamidi, Salim S.
Migdadi, Husayn M.
al-Harti, Ihab Hilmi
al-Fayfi, Sulayman A.
Khan, Muhammad A.
Source
Saudi Journal of Biological Sciences
Issue
Vol. 22, Issue 3 (30 Sep. 2015), pp.340-350, 11 p.
Publisher
Publication Date
2015-09-30
Country of Publication
Saudi Arabia
No. of Pages
11
Main Subjects
Topics
Abstract EN
Forty faba bean (Vicia faba L.) genotypes were evaluated for their agro-morphological performance and molecular diversity under Central Region of Saudi Arabia conditions during 2010–11 and 2011–12 seasons.
Field performance results showed that faba genotypes exhibited a significant amount of variation for their agro-morphological studied parameters.
Giza40 recorded the tallest genotype (139.5 cm), highest number of seeds per plants (100.8), and the highest seed yield per plant (70.8 g).
The best performing genotypes were Giza40, FLIP03-014FB, Gazira1 and Goff1.
Genetic variability among genotypes was determined using Sequence Related Amplified Polymorphism (SRAP) and Amplified Fragment Length Polymorphism (AFLP) markers.
A total of 183 amplified fragments (alleles) and 1758 polymorphic fragments (bands) in SRAP and 202 alleles and 716 bands in AFLP were obtained using six SRAP and four AFLP primer combinations respectively.
Polymorphism information content (PIC) values for AFLP and SRAP markers were higher than 0.8, indicating the existence of a considerable amount of genetic diversity among faba tested genotypes.
The UPGMA based clustering of faba genotypes was largely based on origin and/or genetic background.
Result of cluster analysis based on SRAP showed weak and not significant correlation while, it was highly significant based on AFLP analysis with agromorphological characters (r = 0.01, p > 0.54 and r = 0.26, p < 0.004 respectively).
Combined SRAP and AFLP markers proved to be significantly useful for genetic diversity assessment at molecular level.
They exhibited high discrimination power, and were able to distinguish the faba bean genotypes with high efficiency and accuracy levels.
American Psychological Association (APA)
Ammar, Mujahid H.& al-Ghamidi, Salim S.& Migdadi, Husayn M.& Khan, Muhammad A.& al-Harti, Ihab Hilmi& al-Fayfi, Sulayman A.. 2015. Assessment of genetic diversity among faba bean genotypes using agro-morphological and molecular markers. Saudi Journal of Biological Sciences،Vol. 22, no. 3, pp.340-350.
https://search.emarefa.net/detail/BIM-567847
Modern Language Association (MLA)
Ammar, Mujahid H.…[et al.]. Assessment of genetic diversity among faba bean genotypes using agro-morphological and molecular markers. Saudi Journal of Biological Sciences Vol. 22, no. 3 (2015), pp.340-350.
https://search.emarefa.net/detail/BIM-567847
American Medical Association (AMA)
Ammar, Mujahid H.& al-Ghamidi, Salim S.& Migdadi, Husayn M.& Khan, Muhammad A.& al-Harti, Ihab Hilmi& al-Fayfi, Sulayman A.. Assessment of genetic diversity among faba bean genotypes using agro-morphological and molecular markers. Saudi Journal of Biological Sciences. 2015. Vol. 22, no. 3, pp.340-350.
https://search.emarefa.net/detail/BIM-567847
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 349-350
Record ID
BIM-567847